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EC number: 916-222-4 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01.05.1995-17.05.1995
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot 408
- Purity: 95%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0, 0.5, 1.0, 50, 500, 5000 mg/L
- Vehicle:
- not specified
- Details on test solutions:
- not specified
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: ceriodaphnia dubia
- Strain/clone: not specified
- Source: not specified
- Age of parental stock (mean and range, SD): not specified
- Feeding during test: not specified
- Food type: not specified
- Amount: not specified
- Frequency: not specified
ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): same
- Type and amount of food: not specified
- Feeding frequency: not specified
- Health during acclimation (any mortality observed): not specified - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Readings taken after 24h due to total mortality.
- Hardness:
- not specified
- Test temperature:
- not specified
- pH:
- between 7.2 and 8.6
- Dissolved oxygen:
- between 8.4 and 8.6 mg/L
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- 0-5000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: not specified
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: not specified
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not specified
- Total organic carbon: not speciifed
- Particulate matter: not specififed
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Conductivity: not specified
- Salinity: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: not specifed
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: not specified
- Observations on body length and weight: not specified
- Other biological observations: not specified
- Mortality of control: not specified
- Other adverse effects control: not spcified
- Abnormal responses: not specified
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not specified
- Effect concentrations exceeding solubility of substance in test medium: not specified - Results with reference substance (positive control):
- - Results with reference substance valid? not specified
- Relevant effect levels: not specified
- Limit test: not specified
- Dose-response test: not specified
- ECx: not specified
- Other: not specified - Reported statistics and error estimates:
- not specified
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test material exhibited extreme toxicity in a 48hour, unaerated, static acute test.
- Executive summary:
The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.
Results:
Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.
The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static avute test using Ceriodaphnia dubia.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01.05.1995-17.05.1995
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot 408
- Purity: 95%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0, 0.5, 1.0, 50, 500, 5000 mg/L
- Vehicle:
- not specified
- Details on test solutions:
- not specified
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnia magna
- Strain/clone: not specified
- Source: not specified
- Age of parental stock (mean and range, SD): not specified
- Feeding during test: not specified
- Food type: not specified
- Amount: not specified
- Frequency: not specified
ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): same
- Type and amount of food: not specified
- Feeding frequency: not specified
- Health during acclimation (any mortality observed): not specified - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Reading taken after 24h exposure due to total mortality.
- Hardness:
- not specified
- Test temperature:
- not specified
- pH:
- 7.0-8.3
- Dissolved oxygen:
- 8.7-8.8
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- not specified
- Details on test conditions:
- TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: not specified
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: not specified
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not specified
- Total organic carbon: not speciifed
- Particulate matter: not specififed
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Conductivity: not specified
- Salinity: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: not specified
- Observations on body length and weight: not specified
- Other biological observations: not specified
- Mortality of control: not specified
- Other adverse effects control: not spcified
- Abnormal responses: not specified
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not specified
- Effect concentrations exceeding solubility of substance in test medium: not specified - Results with reference substance (positive control):
- - Results with reference substance valid? not specified
- Relevant effect levels: not specified
- Limit test: not specified
- Dose-response test: not specified
- ECx: not specified
- Other: not specified - Validity criteria fulfilled:
- yes
- Conclusions:
- The test material exhibited extreme toxicty in a 48-hour, unaerated, static acute test using Daphnia magna.
- Executive summary:
The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.
Results:
Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.
The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static acute test using Daphnia magna.
Referenceopen allclose all
Dissolved oxygen and pH:
| At Test Initiation | At Test Completion | |||||||
| pH | Dissolved Oxygen (mg/L) | pH | Dissolved Oxygen (mg/L) | |||||
| Replicate | Replicate | Replicate | Replicate | |||||
| Nominal concentration replicate (mg/L) | A | B | A | B | A | B | A | B |
| 0 | 8.5 | 8.5 | 8.6 | 8.6 | 8.2 | 8.2 | 8.6 | 8.6 |
| 0.5 | 8.5 | 8.5 | 8.6 | 8.6 | 8.2 | 8.2 | 8.6 | 8.6 |
| 1 | 8.6 | 8.6 | 8.6 | 8.5 | 8.3 | 8.3 | 8.6 | 8.6 |
| 50 | 8.6 | 8.6 | 8.5 | 8.5 | 8.3 | 8.3 | 8.6 | 8.6 |
| 500 | 8.4 | 8.5 | 8.5 | 8.5 | 8.3 | 8.3 | 8.6 | 8.6 |
| 5000 | 7.2 | 7.2 | 8.6 | 8.6 | 7.7 | 7.7 | 8.4 | 8.4 |
Reading taken approximately 24 hours after exposure due to total mortatility.
Dissolved Oxygen and pH:
| At Test Initiation | At Test Completion | |||||||
| pH | Dissolved Oxygen (mg/L) | pH | Dissolved Oxygen (mg/L) | |||||
| Replicate | Replicate | Replicate | Replicate | |||||
| Nominal concentration replicate (mg/L) | A | B | A | B | A | B | A | B |
| 0 | 8.3 | 8.3 | 8.7 | 8.7 | 8.3 | 8.3 | 8.7 | 8.7 |
| 0.5 | 8.3 | 8.3 | 8.7 | 8.7 | 8.3 | 8.3 | 8.7 | 8.7 |
| 1 | 8.3 | 8.3 | 8.7 | 8.7 | 8.3 | 8.3 | 8.7 | 8.7 |
| 50 | 8 | 8 | 8.7 | 8.7 | 8 | 8 | 8.7 | 8.7 |
| 500 | 7.5 | 7.5 | 8.7 | 8.7 | 7.8 | 7.8 | 8.7 | 8.7 |
| 5000 | 7 | 7 | 8.7 | 8.7 | 7.4 | 7.4 | 8.8 | 8.8 |
Reading taken approximately 24 hours after exposure due to total mortality.
Description of key information
The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.
Results:
Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.
The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static avute test using Ceriodaphnia dubia.
The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.
Results:
Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.
The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static acute test using Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
