N-methyldiallylamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21-Dec-2017 to 18-Jan-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-methyldiallylamine

EC Number:

219-354-4

EC Name:

N-methyldiallylamine

Cas Number:

2424-01-3

Molecular formula:

C7H13N

IUPAC Name:

methylbis(prop-2-en-1-yl)amine

Specific details on test material used for the study:

- Source and lot/batch No.of test material: Lot 20160912
- Purity: 99.2%
- Expiration date of the lot/batch: 1-August-2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: test substance stable

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Activated sludge from the 'Waterschap Aa en Maas' municipal sewage treatment plant ('s-Hertogenbosch, The Netherlands), receiving predominantly domestic waste. The sludge was diluted slightly with mineral medium, and the concentration of suspended solids was determined to be 4.0 g/L as used for the test.
- Storage conditions: Continuous aeration until use.
- Storage length: Not reported ("freshly obtained")
- Preparation of inoculum for exposure: Sludge allowed to settle 30 minutes and liquid decanted for use as inoculum.
- Pretreatment: None
- Concentration of sludge: 10 mL/L mineral medium

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

other:

Remarks:

organic carbon

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral medium as per method TG 310, water was purified by reverse osmosis and subsequently passed over activated carbon and ion-exchange cartridges (Millipore, Bedford, MA, USA)
- Solubilising agent: none
- Test temperature: 19 - 20 °C
- pH: 7.7 at beginning of test
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: septum-sealed glass bottles, 160 mL capacity
- Number of culture flasks/concentration: triplicate bottles for days 1, 7, 14, and 21, five bottles for day 28, plus three parallel reserve vessels
- Method used to create aerobic conditions: Test was performed in sealed bottles with a headspace: medium ratio of 1:2 to provide a reservoir of oxygen adequate for the duration of the exposure.
- Measuring equipment: Equipment as detailed
- Test performed in closed vessels due to significant volatility of test substance: Yes
- Details of trap for CO2 and volatile organics if used: Closed bottle test. Evolved CO2 was forced into the test medium by the addition of 1 mL 7M NaOH.
SAMPLING
- Sampling frequency: Days 1, 7, 14, 21, and 28
- Sampling method: bottles sacrificed and analyzed as detailed
- Sterility check if applicable: None
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, sampled at each time point
- Abiotic sterile control: yes, inoculum free sample additionally treated with 1 mL/L of a 10 g/L solution of HgCl2 (sampled on Days 1, and 28 only)
- Toxicity control: Yes, reference substance and MTDID 49220, each at 20 mg C/L (sampled on Days 1, 7, and 14 only)
- Positive control: Sampled on Days 1, 7, and 14 only

Reference substance

Reference substance:

other:

Remarks:

Octan-1-ol (CAS No. 111-87-5)

Results and discussion

% Degradation

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

ca. 0

Sampling time:

28 d

Remarks on result:

other:

Remarks:

Not significantly different from blanks

Details on results:

Average inorganic carbon (IC) concentration in blanks (Table 1) on Day 28 was 0.8838 mg/L (SD, 0.181 mg/L). Average IC concentration in MTDID 49220 bottles (Table 2) on Day 28 was 0.8968 mg/L (SD, 0.045 mg/L), corresponding to 0% degradation.
The reference substance was 64% degraded by day 14 (Table 3). The toxicity control (Table 3) exhibited 29% degradation on Day 14, thus exceeding the criteria of greater than 25% degradation by Day 28, and thus MTDID 49220 was considered to have not inhibited microbial activity in this study.

Any other information on results incl. tables

Table 1, Individual concentrations of inorganic carbon (IC) in mg/L for the blank control during the biodegradation test.

Day	Vessel A	Vessel B	Vessel C	Vessel D	Vessel E	Mean	Std. Dev.
1	0.9454	1.022	0.8558	n.a.	n.a.	0.9411	0.083
7	0.7469	0.8846	0.9757	n.a.	n.a.	0.8691	0.115
14	0.8836	1.044	1.076	0.9720	1.222	1.040	0.126
22	1.082	0.8734	0.8760	n.a.	n.a.	0.9438	0.120
28	3.091*	0.8108	0.7392	0.8372	1.148	0.8838	0.181

n.a. - not applicable (not measured)

* - outlier according to Dixon's Q-test; value is not used in further calculations

Table 2, Individual concentrations of inorganic carbon (IC) in mg/L for MTDID 49220 during the biodegradation test.

Day	Vessel A	Vessel B	Vessel C	Vessel D	Vessel E	Mean	Std. Dev.	Percent Degradation
1	0.8749	1.124	1.085	n.a.	n.a.	1.0280	0.134	0
7	1.120	1.034	1.062	n.a.	n.a.	1.0720	0.044	1
14	1.237	1.286	0.8520	n.a.	n.a.	1.125	0.238	0
22	1.474	0.9756	1.022	n.a.	n.a.	1.1572	0.275	1
28	0.9761	0.8818	0.8759	0.8701	0.8801	0.8968	0.045	0

Table 3, Individual concentrations of inorganic carbon (IC) in mg/L for positive control (R), toxicity control (T), and abiotic control (A) during the biodegradation test.

Day	Vessel A	Vessel B	Vessel C	Vessel D	Vessel E	Mean	Std. Dev.	Percent Degradation
R1	0.8717	1.072	1.261	n.a.	n.a.	1.0682	0.195	1
R7	9.614	11.42	11.19	n.a.	n.a.	10.7413	0.983	49
R14	16.1	13.08	14.38	14.39	10.99	13.788	1.897	64
T1	0.8454	1.198	1.206	n.a.	n.a.	1.0831	0.206	0
T7	10.09	7.308	9.009	n.a.	n.a.	8.8023	1.402	20
T14	10.98	15.02	12.29	12.10	13.60	12.7980	1.552	29
A1	0.9432	0.9523	0.7365	n.a.	n.a.	0.8773	0.122	0
A28	0.6316	0.4465	0.7861	0.4906	0.4063	0.5522	0.156	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

less than 3 mg/L inorganic carbon was in blanks at end of test, reference substance greater than 60% degraded by day 14

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

MTDID 49220 is not readily biodegradable, as determined according to OECD TG 310.

Executive summary:

Ready biodegradability of MTDID 49220 was studied in a test done according to OECD TG 310. MTDID 49220, octan-1-ol (reference substance), or both chemicals (toxicity control) were introduced to test bottles at 20 mg/L theoretical organic carbon (ThOC). Bottles also contained mineral medium inoculated with activated sludge from a predominantly domestic source. Blank bottles containing only inoculated medium were also prepared. Bottles were sacrificed at intervals by addition of 7M NaOH to draw evolved CO2 into the mineral medium. After equilibration, CO2 concentrations were measured using a carbon analyzer.

No degradation of MTDID 49220 was measured after 28 days of incubation. The blank bottles had < 3 mg/L inorganic carbon, and the reference substance was >60% degraded by day 14. The toxicity control exhibited sufficient degradation (>25% by 14 days) that MTDID 49220 was considered to have not inhibited microbial actitivity in this test. Since MTDID 49220 was not biodegraded in this test, MTDID 49220 is not readily biodegradable. This test was conducted under an internationally accepted guideline for volatile chemicals, and was in accord with GLP criteria. Therefore, this study is reliable without restrictions.