Titanium 2,2',2''-nitrilotrisethanolate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Justification for type of information:

Read across

Cross-reference

Reason / purpose for cross-reference:

read-across source

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Test item concentration not in accordance to guideline.

Justification for type of information:

Read across

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Continuous biological activated sludge treatment system

Principles of method if other than guideline:

Continuous biological activated sludge treatment system

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material (as cited in study report): Methyldiethanolamine (MDEA; CAS: 105-59-9)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from municipal wastewater treatment plant (TOC: 1920 mg/l, total solids (TS): 9.4 g/l)

Parameter:

% degradation (TOC removal)

Value:

> 91

Sampling time:

21 d

Details on results:

- start-up period: ca. 2 weeks;
degradation for TOC of influent > 95 % - 80 ml/d MDEA (40 %): rise of TOC in effluent, increase of
degradation after 2 weeks - 40 ml/d MDEA (40 %): recovery after reduction of MDEA load; 95 % TOC degradation - MDEA-solution easily eliminated after adaption of the sludge: up to 96 % TOC-removal

- The continuous flow experiment showed a very good biodegradability of the wastewater including for high concentrations of MDEA solution with a TOC removal of 96%.

Conclusions:

- very good biodegradability of the wastewater including for
high concentrations of MDEA-solution (TOC removal: 96 %)

- high biodegradation probably due to adaptation of
microorganisms

Data source

Reference

Reference Type:

publication

Title:

Biodegradation of triethanolamine.

Author:

West RJ and Gonsior SJ

Year:

1996

Bibliographic source:

Environ Toxicol Chem 15(4): 472-480.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: CO2 evolution test

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,2',2''-nitrilotriethanol

EC Number:

203-049-8

EC Name:

2,2',2''-nitrilotriethanol

Cas Number:

102-71-6

Molecular formula:

C6H15NO3

IUPAC Name:

2,2',2''-nitrilotriethanol

Specific details on test material used for the study:

- Name of test material (as cited in study report): [14C]-triethanolamine
- Analytical purity: >= 98 %
- Specific activity (if radiolabelling): 17.61 mCi/mmol

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixed liquor activated sludge (secondary effluent)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): West Bay County WWTP, Bay City, Michigan, treating primarily municipal sewage up to 4 mio gallons per day

Duration of test (contact time):

6 d

Initial test substance concentration

Initial conc.:

> 600 - < 5 500 µg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: minimal salts basal medium according to Stainer et al. (1966; J Gen Microbiol 43: 159-271)
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 25 °C
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes
- Other:

TEST SYSTEM
- Culturing apparatus: 500-mL polypropylene Erlenmeyer flasks, sealed with rubber stoppers and fitted with CO2 traps
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used: glass tubular CO2 traps (4 mL 1N KOH)
- Other: test material amended with 14C-TEA to yield 0.7 µCi/mL

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control: yes, via addition of formaldehyde (final concentration: 2.8%)
- Toxicity control:
- Other: performed in duplicate

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (test mat. analysis)

Value:

ca. 100

Sampling time:

1 d

Remarks on result:

other: Primary biodegradation of 0.6 mg/l TEA in a test with 164 mg/l municipal activated sludge

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Value:

ca. 100

Sampling time:

5 d

Remarks on result:

other: Primary biodegradation of 0.6 mg/l TEA in a test with 164 mg/l municipal activated sludge

Details on results:

The rate constants in all test batches for degradation and mineralization are >0.359. According to GHS legislation, the substance is rapidly biodegradable. Therefore it can be assumed that the substance is also readily biodegradable.

Any other information on results incl. tables

Biodegradation (activated sludge):

Activated sludge (mg/L MLSS)	Test item concentration (mg/L)	Biodegradation rate constant (/d)	Biodegradation half-life (d)	CO2/ThCO2 (%)
164	0.6	6.86	0.1	56+-10
164	5.7	1.36	0.51	54+-3
818	0.6	30	0.02	55+-1
818	5.7	6.7	0.01	52+-0

 

Mineralisation (activated sludge):

Activated sludge (mg/L MLSS)	Test item concentration (mg/L)	Mineralisation rate constant (/d)	Mineralisation half-life (d)
164	0.6	1.92	0.36
164	5.7	1.04	0.67
818	0.6	2.86	0.24
818	5.7	1.69	0.41

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: rapidly biodegradable according to GHS (readily biodegradability is therefore assumed)