Titanium 2,2',2''-nitrilotrisethanolate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study meets generally accepted scientific principles, well documented, according to national australian guideline wich is comparable to more widespread used guidelines. acceptable for assessment.

Justification for type of information:

Read across

Qualifier:

according to guideline

Guideline:

other: New South Wales Gouvernment Environment Protection Authority: ASTM Designation E1192 (1988)

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Triethanolamine
- Analytical purity: no data
- stored in the dark at 22±2 °C

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions, stirred in the dark; diluted to the appropriate concentrations immediately prior to the commencement of the test.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

TEST ORGANISM
- Common name: Ceriodaphnia cf. dubia
- Source: laboratory
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: cultures were maintained in 2-L glass beakers and transferred to fresh water three times weekly. Food was provided after water renewal (unicellular algae: Pseudokircheneriella subcapitata and Ankistrodesmus sp., each at 25000 cells/mL)
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

23±1 °C

Nominal and measured concentrations:

series of 5 test concentrations plus control

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Material, size, headspace, fill volume: 250-mL glass beakers, fill volume: 200 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 1 animal / 40 mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tapwater, filtered (1 µm), aged (1 month), adjusted to 500 µS/cm with seawater


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h light:dark cycle
- Light intensity: 1000 lux at surface

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- measurements:
- number of immobile cladocera counted at the end of test
- pH, dissolved oxygen, conductivity at test initiation before test animals were added and at test completion

TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: performed
- Test concentrations: no data

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

609.88 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence interval 565.2 mg/l - 658.3 mg/l

Remarks:

The 48-h EC50 (immobilisation) values and the corresponding 95 % confidence intervals were based on nominal concentrations and were determined by the trimmed Spearman-Kaerber method (Hamilton et al., 1977).

Validity criteria fulfilled:

yes

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

RA

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Annual Book of ASTM Standards: Water and Environmental Technology. Vol 1104, (1994)

Principles of method if other than guideline:

Annual Book of ASTM Standards: Water and Environmental Technology. Vol 1104, (1994)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

80-100 mg/l CaCO3

Test temperature:

20.5 -22C

pH:

8.1-9.4

Dissolved oxygen:

7.6 - 8.5

Nominal and measured concentrations:

31.2, 62.5, 125, 250, 500 mg/l

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

230 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Conclusions:

48 h LC50= 233 mg/L (nominal; static)
48 h LC50= 230 mg/L (nominal; static)

Description of key information

Testing was considered not necessary as alternatives to testing were available and they demonstrated to be sufficient to fulfil the legal requirements related to Annex VII, Section 9.1.1 of REACH. Therefore, the general rules for adaptation 1.2 of Annex XI of REACH regulation are met and applied. The results obtained from the three publications available from were in the EC50 range 609 -125 mg/l. the EC50 of 125 mg/l has been chosen as key for the CSA.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

125 mg/L