N,N-bis(2,3-epoxypropyl)aniline

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study performed according to OECD Guideline 203 [1992] and EEC directive 92/69 Part C.1 [1992]. No analytical control of test concentrations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline

EC Number:

225-716-2

EC Name:

p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline

Cas Number:

5026-74-4

Molecular formula:

C15H19NO4

IUPAC Name:

4-(oxiran-2-ylmethoxy)-N,N-bis(oxiran-2-ylmethyl)aniline

Test material form:

liquid: viscous

Details on test material:

- Substance type: organic
- Storage condition of test material: In refrigerator (2-8°C) in the dark
- Stability under test conditions: stable
- Descrition : Clear yellow liquid (Determined at NOTOX)
- Analytical purity: >95%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material dissolved by stirring in acetone to create stock solutions. 0.2 ml of solvent was added to 2 l of test medium and mixed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.01%
- Evidence of undissolved material (e.g. precipitate, surface film, etc): All final test solutions appeared clear and without precipitation.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp (Cyprinus carpio. Teleostei, Cyprinidae) (Linnaeus, 1758)
- Reason for selection : This system has been selected as an internationally accepted species.
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, NL
- Total fish used : 79
- Length at study initiation (length definition, mean, range and SD): 1.96 +/- 0.10 cm
- Weight at study initiation (mean and range, SD): 0.21 +/- 0.03 g
- Method of breeding: F1 from a single partent-pair bred in UV-treated water
- Feeding during test : no feeding from 24 hours prior to test and during total test period

ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same, with feeding; test without feeding
- Type and amount of food: Trouvit, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): < 5% mortality during the 7 days prior the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

20.5 deg C

pH:

7.6 - 8.1

Dissolved oxygen:

9.2 - 8.7 mg/L

Salinity:

Iso medium (Ca++, Mg++, Na+, K+, Cl-, SO4 2-, HCO3-); total 351 mg / L

Nominal and measured concentrations:

Nominal: 10, 5.6, 3.2, 1.8, 1.0 mg/L test material plus a solvent and a negative control
Analytics have not been performed

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 liters
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass
- Aeration: continously
- No. of organisms per vessel: 7 fish per concentration and control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.74 g fish / litre, e.g. 7 fish per 2 litres of test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Millipore reverse osmosis tap water, supplemented with salts to generate iso medium
- Particulate matter: none observed
- Intervals of water quality measurement: Oxygen, pH, temperature: daily // Ammonia, Nitrite, Nitrate once a week.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours / day

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality (at 2, 24, 48, 72 and 96 hours after start of treatment)
- disturbance of swimming behaviour (at 2, 24, 48, 72 and 96 hours after start of treatment)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L in Rang-Finding study
- Results used to determine the conditions for the definitive study: 100% mortality at 100 and 10 mg/L, 0% mortality at 1 mg/L in RF study

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

>= 5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

<= 3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: Swimming behaviour disturbed at 48 hours of 3 moribund fish at the concentration of 5.6 mg/l
- Observations on body length and weight: no data
- Other biological observations: no data
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no observations
- Effect concentrations exceeding solubility of substance in test medium: no data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 in carp after 96 hours of treatment is 4.2 mg/L with 0% mortality at 3.2 mg/L and 100% mortality at 5.6 mg/L.

Executive summary:

In the acute toxicity study in carp, each 7 fish were exposed for 96 hours to diluted solutions of TK 12576. Acetone was used as a solvent at 0.01% in the test medium. The concentration range tested was selected on the basis of results obtained in a range finder study. The LC50 after 96 hours of treatment was 4.2 mg/L with 0% mortality at 3.2 mg/L and 100% mortality at 5.6 mg/L.