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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21.03.2018 - 18.04.2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Deviations:
no
GLP compliance:
no
Remarks:
accredited by DAkkS

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dihydroxybenzoic acid
EC Number:
201-946-9
EC Name:
2,4-dihydroxybenzoic acid
Cas Number:
89-86-1
Molecular formula:
C7H6O4
IUPAC Name:
2,4-dihydroxybenzoic acid
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, non-adapted
Duration of test (contact time):
28 d

Results and discussion

% Degradation
Key result
Parameter:
% degradation (DOC removal)
Value:
100
Sampling time:
28 d

Any other information on results incl. tables

Table 1: DOC-content of blank value-samples and samples with 2,4-dihydroxybenzoic acid, calculated mean values and resulting difference as well as DOC-degradation within total test duration.

test duration [d]

DOC [mg/l] of 2,4-Dihydroxybenzoic acid-samples

DOC [mg/l] of blank value-samples

DOC-difference [mg/l]

DOC degradation [%]

I

II

mean

I

II

mean

0

45,64

43,26

44,45

6,19

0,50

3,35

41,11

0,00

2

46,16

45,65

45,91

2,04

1,43

1,74

44,17

0,00

5

43,58

41,24

42,41

3,20

0,89

2,05

40,37

1,81

8

< 0,5

< 0,5

< 0,5

7,00

4,29

5,65

-5,15

100

13

< 0,5

< 0,5

< 0,5

3,69

8,72

6,21

-5,71

100

16

1,77

0,50

1,14

0,56

3,31

1,94

-0,80

102

19

5,77

5,25

5,51

4,15

4,75

4,45

1,06

97,4

21

5,86

5,38

5,62

3,82

6,61

5,22

0,41

99,0

23

8,41

5,53

6,97

6,32

7,28

6,80

0,17

99,6

26

5,86

8,38

7,12

8,58

7,14

7,86

-0,74

102

28

8,08

7,37

7,73

7,94

7,53

7,74

-0,01

100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The reference samples biodegraded within 2 days to a degree of 76%. Abiotic control samples showed only low DOC-degradation of 44 mg/l to 38 mg/l.
Interpretation of results:
readily biodegradable
Conclusions:
2,4-dihydroxybenzoic acid is rapidly biodegradable as deteminend by a OECD 301A: DOC Die-Away-Test.
Executive summary:

As a criterion for the classification as rapidly biodegradable a DOC-elimination of 70% of the test item within a 10 day-window during a total test duration of 28 days is considered.

The elimination rate in the degradation phase was calculated from measured data of the 5th and 8th day to be 32,7% of DOC per day.The 70%- degradation level in the 10 day-window is clearly exceeded.

Hence, 2,4-dihydroxybenzoic acid is classified as rapidly biodegradable.