Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labeling and/or risk assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Remarks:
The study was conducted according to the test guidelines in effect at the time of study conduct.
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Purity: not reported
Radiolabelling:
no

Study design

Batch equilibrium or other method

Analytical monitoring:
yes
Details on sampling:
Reference substances included acetanilide, aniline, benzamide, benzoic acid phenyl ester, 2,5-dichloroaniline, 4-nitrobenzamide, phenanthrene, and urea. Reference stock solutions were prepared by weighing the following amounts of each references substance into individual 10-mL volumetric flasks and diluting to volume with methanol. The stock solutions were sonicated as necessary to dissolve the reference substances. Three combined calibration solutions were prepared by transferring aliquots from each of the reference stock solutions in three 10-mL volumetric flasks. Standard solutions were diluted to volume with 55%/45% methanol/water. Segregation of reference substances was determined to avoid potential retention time overlap. Aliquot size was determined to control peak height. The test substance was prepared by weighing 38.9 mg of the test substance into a 10 mL volumetric flask and diluting to volume with 55%/45% methanol/water. The calibration standards and samples preparations were analyzed in triplicate by liquid chromatography with ultraviolet spectroscopic detection.

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
12
Remarks on result:
other: soil
Type:
log Koc
Value:
1.08
Remarks on result:
other: soil
Type:
Koc
Value:
12.6
Remarks on result:
other: sludge
Type:
log Koc
Value:
1.1
Remarks on result:
other: sludge

Results: Batch equilibrium or other method

Transformation products:
not specified

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Log Koc: Soil (1.08), Sludge (1.10)
Koc : Soil (12.0), Sludge (12.6)
This study and the conclusions which are drawn from it fulfill the quality criteria (validity, reliability, repeatability).
Executive summary:

Estimates of the adsorption coefficient (Koc) for the test substance on soil and sludge were established employing methodology described in the OECD Guidelines 121: Estimation of the Adsorption Coefficient (Koc) on Soil and Sewerage Sludge using High Performance Liquid Chromatography. The Koc and log Koc for soil were 12.0 and 1.08, respectively. The Koc and log Koc for sludge were 12.6 and 1.10, respectively.