[No public or meaningful name is available]

Long-term toxicity to fish

Administrative data

Endpoint:

fish short-term toxicity test on embryo and sac-fry stages

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 2010 - January 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Guidelines for testing of chemicals, No. 212

Version / remarks:

China Environmental Science Press, 2004

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)

Version / remarks:

Adopted September 21st, 1998

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Cas Number:

890707-29-6

Molecular formula:

C10H11N3O

IUPAC Name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: Tuebingen strain
- Source: Minghong Aquarium, Hangzhou, Zhejiang Province

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

8 d

Test conditions

Test temperature:

20-22°C

pH:

7.0-7.3

Dissolved oxygen:

> 60% of air saturation value

Nominal and measured concentrations:

10.5 mg/L (nominal), 9.24 mg/L (average of measured concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: Petri dish
- Aeration: No
- Renewal rate of test solution: At day 3 and day 6
- No. of fertilized eggs/embryos per vessel: 30
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 14 h light/10 hour dark
- Light intensity: 1800-2300 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Temperature, pH, dissolved oxygen content

VEHICLE CONTROL PERFORMED: no

Results and discussion

Effect concentrations

Key result

Duration:

8 d

Dose descriptor:

LC50

Effect conc.:

> 10.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No poisoning symptoms for embryos and sac-fries observed during study.

Any other information on results incl. tables

Table 1: Survival of zebra fish embryos and fries in control.

Time of exposure (d)			1	2	3	4	5	6	7	8
Replicate	Number of test embryo		Accumulated number								Embryo hatch %	Embryo death %	Fry death %
1	30	Embryo hatch	0	1	3	27	27	27	27	27	90.0	10.0	0
		Embryo death	1	1	1	1	3	3	3	3
		Fry death	-	0	0	0	0	0	0	0
2	30	Embryo hatch	0	6	11	28	28	28	28	28	93.3	6.7	0
		Embryo death	1	1	2	2	2	2	2	2
		Fry death	-	0	0	0	0	0	0	0
3	30	Embryo hatch	0	4	9	27	27	27	27	27	90.0	10.0	0
		Embryo death	0	2	2	3	3	3	3	3
		Fry death	-	0	0	0	0	0	0	0

 

 

Table 2: Survival of zebra fish embryos and fries in test concentration.

Time of exposure (d)			1	2	3	4	5	6	7	8
Replicate	Number of test embryo		Accumulated number								Embryo hatch %	Embryo death %	Fry death %
1	30	Embryo hatch	0	1	2	27	27	27	27	27	90.0	10.0	0
		Embryo death	1	1	2	3	3	3	3	3
		Fry death	-	0	0	0	0	0	0	0
2	30	Embryo hatch	0	4	9	27	28	28	28	28	93.3	6.7	0
		Embryo death	1	2	2	2	2	2	2	2
		Fry death	-	0	0	0	0	0	0	0
3	30	Embryo hatch	0	2	8	28	28	28	28	28	93.3	6.7	0
		Embryo death	1	2	2	2	2	2	2	2
		Fry death	-	0	0	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Result showed that the test substance did not have any obvious lethal hazards to embryos and sac-fries of the fish at nominal concentration of 10.5 mg/l.

Executive summary:

The toxicity of the test substance to embryos and sac-fries of the zebra fish Brachydanio rerio was investigated according to OECD Guideline 212.

Under the test conditions, no lethal hazards to embryos and sac-fries were observed at a nominal concentration of 10 g test material/L.