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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 1997 - August 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Principles of method if other than guideline:
The test was performed in accordance with Notice “Test methods for new chemical substances –
Biodegradability test of chemical substances by microorganisms, etc.” specified in “Kan-Ho-gyo
No. 5”, “Yaku-Hatsu No. 615” and “49Ki-Kyoku No. 392” issued in1972.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Trehalose
EC Number:
202-739-6
EC Name:
Trehalose
Cas Number:
99-20-7
Molecular formula:
C12H22O11
IUPAC Name:
trehalose
Test material form:
solid: crystalline
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 609111
- Expiration date of the lot/batch: not stated
- Purity test date: not stated

RADIOLABELLING INFORMATION (if applicable)
not applicable

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not stated
- Stability under test conditions: assumed stable
- Solubility and stability of the test substance in the solvent/vehicle: assumed stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: assumed stable

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
not specified

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Standard activated sludge purchased from Chemicals Inspection &
Testing Institute, Japan (now Chemicals Evaluation and Research
Institute, Japan), Chemicals Safety Center on July 24, 1997 was used
for the test.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
DOC removal
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
TEST CONDITIONS
- Composition of medium: ml of A, B, C and D solutions specified in JIS K
0102, 21(1) was added to 1976 mL of deionized water
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 25 +- 1 °C
- pH: 7
- pH adjusted: yes
- CEC (meq/100 g): not stated
- Aeration of dilution water: not stated
- Suspended solids concentration: not stated
- Continuous darkness: not stated
- Other: not stated

TEST SYSTEM
- Culturing apparatus: not stated
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: not stated
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: no further equipment during test
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: not applicable
- Other:

SAMPLING
- Sampling frequency: weekly
- Sampling method: by device (Ohkura Electric Co., Ltd.
“Coulometer OM-3001A”)
- Sterility check if applicable: not specified
- Sample storage before analysis: not specified
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
- Other:

STATISTICAL METHODS: none applied
Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
none
Test performance:
No information reported on performance of test
% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
>= 68 - <= 74
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
>= 62 - <= 67
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
>= 57 - <= 60
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
>= 47 - <= 49
Sampling time:
7 d
Key result
Parameter:
% degradation (DOC removal)
Value:
>= 98 - <= 100
Sampling time:
28 d
Parameter:
% degradation (test mat. analysis)
Value:
100
Sampling time:
28 d
Details on results:
Direct measurement of Trehalose in blank control by HPLC showed 101% recovery at day 28.

Any other information on results incl. tables

 

BiodegradabilitycalculatedfromBOD

Culture Bottle No.

Items

Theoretical

oxygen

demand

(mg)

Day 7

Day 14

Day 21

Day 21

Measured

value (mg)

Degradability

(%)

Measured

value (mg)

Degradability

(%)

Measured

value (mg)

Degradability

(%)

Measured

value (mg)

Degradability

(%)

1

Test article section

1

 

33.7

 

19.6

 

47

 

24.3

 

57

 

26.7

 

62

 

29.3

 

68

2

Test article section

2

 

33.7

 

19.9

 

48

 

24.6

 

58

 

28.5

 

67

 

32.5

 

78

3

Test article section

3

 

33.7

 

20.4

 

49

 

25.1

 

60

 

28.3

 

67

 

31.2

 

74

4

Aniline

section

 

90.8

 

53.3

 

55

 

70.3

 

72

 

72.3

 

73

 

72.8

 

73

5

Basicbreathing

section

-

3.8

-

5.0

-

5.8

-

6.3

-

 

6

Stability

section

 

-

 

0.0

 

-

 

0.0

 

-

 

0.0

 

-

 

0.0

 

-

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable, but failing 10-day window
Conclusions:
As the results calculated from BOD, the test article degradability was 68%, 78% and 74%
(average 73%).
As the results calculated from DOC, the test article degradability was 100%, 97% and 98%
(average 98%).
By HPLC method, the test article detectable at the end of test period,
the degradability of test article was all 100%.
Based on the above results, it was concluded that test article, trehalose, is ready biodegradable
under the conditions specified by Test methods for new chemical substances –
Biodegradability test of chemical substances by microorganisms, etc.” specified in “Kan-Ho-gyo
No. 5”, “Yaku-Hatsu No. 615” and “49Ki-Kyoku No. 392” issued in1972.