Pyrimidine-2,4,6-triyltriamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non-GLP screening study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

This study is a screening test and does not include all aspects required by the standard test guidelines.
Main modifications:
- Reduced number of test concentrations (3) with a spacing factor of 10
- No concentration control analysis
- No statistical evaluation

GLP compliance:

no

Remarks:

This study does not have a GLP status. However, the experimental conduct of this study was carried out in general in accordance with the OECD GLP Principles, the GLP Principles of the German Chemical Act and, as far as applicable, by laboratory SOPs.

Test material

Test material information

Constituent 1

Reference substance name:

Pyrimidine-2,4,6-triyltriamine

EC Number:

213-720-7

EC Name:

Pyrimidine-2,4,6-triyltriamine

Cas Number:

1004-38-2

Molecular formula:

C4H7N5

IUPAC Name:

2,4,6-Triaminopyrimidine

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Test substance preparation:
Stock solution: 100.2 mg of the test substance were dissolved with M4 media to 1L. The stock solution appeared clear and colorless (correspond to test
concentration of 100 mg/L).
Test solution 1: 10 mL of the stock solution were diluted with M4 media to 1 L (correspond to test concentration of 10 mg/L).
Test solution 2: 10 mL of the test solution 1 were diluted with M4 media to 1 L (correspond to test concentration of 1 mg/L).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna STRAUS, clone 5
- Age at study initiation (mean and range, SD): < 24hours old

- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Source: The D. magna clone used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978 and from this date on was cultured and bred continuously at the test facility.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

20 ± 2°C.

pH:

7.5 – 8.5

Dissolved oxygen:

> 3 mg/L O2

Conductivity:

550 - 650 μS/cm

Nominal and measured concentrations:

0 (negative control), 1, 10, 100 mg/L as nominal concentrations based on the test substance mass.

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 10 mL test solution volume
- Volume of solution: 10 mL test solution volume
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water (Elendt M4)
- Intervals of water quality measurement: Throughout the test, the appearance of the test solutions and dissolution behavior of the test substance was observed and recorded daily. The chemical and physical parameters of the test medium (total hardness, acid capacity, pH, TOC and conductivity) were determined after aeration and prior to use in the test. The pH and dissolved oxygen in the test solutions are recorded at the start and end of the exposure period. Test temperature was maintained at approx. 20 ± 2°C. The pH and the oxygen content range between 8.0-8.1 and 8.3-8.8 mg/L during the exposure.

DATA EVALUATION
This study was designed to provide a qualitative estimate of toxicity. The study design was not sufficient to provide a reliable quantitative toxicity endpoint. The ECx ranges are estimated based on expert judgment in accordance with test guideline recommendations.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The water parameters oxygen content and pH were recorded at start and end of the exposure period and within the ranges (pH 6-9, > 3 mg/L O2) required by the OECD Guideline for Testing of Chemicals No. 202, Daphnia sp., Acute Immobilisation Test.
All test assays were visibly clear and colorless after preparation and remained so after 24 and 48 hours of exposure in the test. No undissolved test substance was visible and there were no other remarkable observations.

Test validity criteria: This test was fully compliant with all the following validity criteria required by the corresponding test guidelines and is considered valid.
- < 10% immobilisation in the control
- O2 concentration > 3 mg/L in control and test vessels

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance is acutely harmful for aquatic invertebrates.