Dipotassium dodecenylsuccinate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-06-26 to 2018-06-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dipotassium dodecenylsuccinate

EC Number:

260-612-0

EC Name:

Dipotassium dodecenylsuccinate

Cas Number:

57195-28-5

Molecular formula:

C16H26K2O4*H2O

IUPAC Name:

Dipotassium dodecenylsuccinate

Test material form:

liquid

Details on test material:

30 % aqueous solution
pure substance is solid (bulk/powder), but is only used as aqueous solution and blends it into mixture
Substance dissolves in water at 40wt%, then becomes gel and becomes solid, if mixed 61wt% with water

Specific details on test material used for the study:

Test Item: Dipotassium dodecenylsuccinate (aq. solution)
Batch number: 170727
Active ingredient: Dipotassium dodecenylsuccinate (30% : Water 70%)
Purity: Active Ingredient 30% - Water 70%
Water solubility: Soluble
Appearance: Light brownish, clear liquid
Stability under under test conditions: Not specified
Expiry date: 2019-07-27
Recommended storage: Store in the thightly closed container in a dark, cool (room temperature), dry and well ventilated place.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Preparation of the test solutions: Five test solutions (100 - 200 - 400 - 800 - 1600 mg/L were weighed out) were individually prepared with ASTM very soft water (acc. to ASTM E729-96 (2002)) prior to the start of the exposure (at 0 hours) as well as prior to the renewal of the test solutions (24 hours). The test solutions were mixed thoroughly by manual agitation. The test solutions were checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative in all concentration levels.

Test concentrations: 5 test item concentrations in a geometric series with a separation factor of 2, were tested as follows: 100 - 200 - 400 - 800 - 1600 mg/L. The test item concentrations were selected based on the results of a non-GLP preliminary range finding test. For results, see Annex I.

Control: Dilution water (as specified in Table 3) without test item incubated under the same conditions as the test groups.
Test method: The study was performed under semi-static conditions, with a renewal of the test solutions after 24 hours.
Test duration: 48 hours
Test vessels: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
Test volume: 20 mL
Dilution water: ASTM very soft water was used with regard to its low hardness. The test item was not soluble in other (harder) waters.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS, obtained from continuous laboratory cultures.
- Justification for species other than prescribed by test guideline: Daphnia magna is the preferred species in accordance with the test guideline and is bred at the test facility.
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD): 2 to 24 hours old daphnids from a healthy stock were used for the study.
- Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 hours illumination; light intensity of max. 1500 lx
- Culture medium: Elendt M4, according to OECD 202, Annex 3 (2004), is used.
- Feeding during test: yes
- Food type: mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: algae cell density of > 10E6 cells/mL.
- Frequency: The culture daphnids were fed at least 5 times per week ad libitum.

ACCLIMATION
- Acclimation period: At least 2 hours in the dilution water

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

21 - 24 mg CaCO3/L

Test temperature:

19.5 - 19.6

pH:

7.92 - 8.46

Dissolved oxygen:

8.65 - 9.32

Conductivity:

39 - 41 µS/cm

Nominal and measured concentrations:

nominal concentrations: 100 - 200 - 400 - 800 - 1600 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses.
- Type: closed
- Renewal rate of test solution (frequency/flow rate): The study was performed under semi-static conditions, with a renewal of the test solutions after 24 hours.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ASTM very soft water was used with regard to its low hardness. The test item was not soluble in other (harder) waters.
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

1 572 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

422 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations of the test item Dipotassium dodecenylsuccinate (aq. solution), the 48 hour-EC50 for Daphnia magna was 422 mg/L (95% confidence limits: 308 – 592 mg/L).