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There is no experimental data available for Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, 2-25% aromatics. Data is read across from structural analogues such as Hydrocarbons, C9-C14 aliphatics, 2-25% aromatics and Hydrocarbons, C14-C20 aliphatic, 2-30% aromatics. Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) straddles Hydrocarbons, C9-C14, Aliphatics, (2-25% Aromatics) and Hydrocarbons, C14-C20, Aliphatics (2-30% Aromatics). Read across justification documents have been provided for the same in Section 13 of the dossier. For this substance, a worst case scenario approach has been used for each endpoint.

 

Acute Fish Toxicity:

Water accommodated fractions of Hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2 -25%), produced a 96-hour LL50 value in the range 10-30 mg/L with rainbow trout, Oncorhynchus mykiss.

 

Water accommodated fractions of Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2 -25%), produced a 96-hour LL50 value in the range 10-100 mg/L with rainbow trout, Oncorhynchus mykiss.

 

Water accommodated fractions of hydrocarbons, C16 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not cause acute toxicity to fish at a nominal loading of 1000 mg/L with rainbow trout, Oncorhynchus mykiss.

The estimated freshwater fish 96 hr LL50 value for Hydrocarbons, C12-C16, n-alkanes,isoalkanes, cyclics, aromatics (2-25%) determined using the Petrotox computer model (HCSC, 2018) is 1117.88mg/L with rainbow trout, Oncorhynchus mykiss based on mortality.

 

Chronic Fish Toxicity:

The aquatic toxicity was estimated using the Petrotox computer model (v. 3.04), which combines a partitioning model used to calculate the aqueous concentration of hydrocarbon components as a function of substance loading with the Target Lipid Model used to calculate acute and chronic toxicity of non-polar narcotic chemicals. Petrotox computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a hydrocarbon substance and membrane-water partitioning coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism.

The estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) determined using the Petrotox computer model (HCSC, 2018) is 0.25 mg/Lwith rainbow trout, Oncorhynchus mykiss, based on growth.

 

The estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) is 0.13 mg/L based on growth.

 

The estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) is 0.091 mg/L based on growth.

 

The estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C11 -C14, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) is 0.079 mg/L based on growth.

 

Hydrocarbons, C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause toxicity to fish in a 21-day toxicity study with Brachydanio rerio following standard test guidelines at a nominal loading of 5000 mg/L. These results indicate that hydrocarbons, C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) is not expected to be chronically toxic to fish.

 

Acute Invertebrate Toxicity:

The estimated aquatic invertebrate 48h EL50 (No Observed Effect Loading Rate) value for Hydrocarbons, C12-C16, n-alkanes,isoalkanes, cyclics, aromatics (2-25%) determined using the Petrotox computer model (CONCAWE, 2010) is >1000 mg/L with Daphnia magna, based on mobility.

Water accommodated fractions of hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, 2-25% aromatics, produced a 48-hour EL50 value in the range 10 - 22 mg/L with Daphnia magna.

 

Water accommodated fractions of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, 2-25% aromatics, produced a 48-hour EL50 value in the range 100 - 220 mg/L with Daphnia magna.

 

Water accommodated fractions of hydrocarbons, C16 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause acute toxicity to the freshwater invertebrate, Daphnia magna at a nominal loading of 1000 mg/L.

 

Chronic Invertebrate Toxicity:

A study was performed on Daphnia magna exposed to hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2-25%). The 21-d NOELR based on reproduction was found to be 0.28 mg/L.

 

Hydrocarbons, C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause toxicity in a 21-day toxicity study with Daphnia magna following standard test guidelines at a nominal loading of 1400 mg/L, with a NOELR of 17 mg/L (based on inhibition of reproduction).These results indicate that hydrocarbons, C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) is not expected to be chronically toxic to freshwater invertebrates.

The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C12-C16, n-alkanes, isoalkanes,cyclics, aromatics (2 -25%) determined using the Petrotox computer model (CONCAWE, 2010) is 0.69 mg/L in Daphnia magna, based on reproduction.

 

The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) determined using the Petrotox computer model (CONCAWE, 2010) is 0.162 mg/L in Daphnia magna, based on reproduction.

 

Algal Toxicity:

Algal cultures were exposed to water accommodated fractions (WAFs) of Hydrocarbons, C9-C12, isoalkanes. cyclics, arornatics (2-25%) in two separate studies. The 72-hr EL50 value for growth rate were found to be 4.1 and 4.6 -10 mg/L. The 72-hr NOELR values for growth rate were 0.76 and 0.22 mg/L, respectively.

 

The EL50 for algal cultures exposed to WAFs of Hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) were 10 -100 mg/L.

 

Water accommodated fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not cause acute toxicity to the freshwater green algae, Pseudokirchneriella subcapitata, at a nominal loading of 1000 mg/L.

 

These data are used as read across data for Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, 2-25% aromatics.

The estimated freshwater algae 72hr EL50 value for Hydrocarbons, C12-C16, n-alkanes, isoalkanes,cyclics, aromatics (2-25%) determined using the Petrotox computer model (CONCAWE, 2010) is 3.05 mg/L in Pseudokirchneriella subcapitata, based on growth.

The estimated freshwater algae 72hr NOEL value for Hydrocarbons, C12-C16, n-alkanes, isoalkanes,cyclics, aromatics (2-25%) determined using the Petrotox computer model (CONCAWE, 2010) is 0.16 mg/L in Pseudokirchneriella subcapitata, based on growth.

 

Microorganisms Toxicity:

The estimated protozoan,Tetrahymena pyriformis, 48-hr EL50 value for Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) is >1000 mg/L determined using the Petrotox computer model (CONCAWE, 2010), based on growth inhibition.

 

The estimated protozoan,Tetrahymena pyriformis, 48-hr NOELR value for Hydrocarbons, C12 -C16, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) is 1117.96 mg/L determined using the Petrotox computer model (CONCAWE, 2010), based on growth inhibition.

The hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2-25%), estimated protozoan,Tetrahymena pyriformis, 48-hr EL50 value is 43.98 mg/L, based on growth inhibition.

 

The hydrocarbons, C11-C14, n-alkanes, isoalkanes, cyclics, aromatics (2-25%), estimated protozoan,Tetrahymena pyriformis, 48-hr EL50 value is 185.1 mg/L, based on growth inhibition.

 

Water Accommodated Fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%), did not inhibit microbial respiration in a sludge sample at a concentration of 1000 mg/L. Therefore, Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%), is not expected to adversely affect microbial activity in a wastewater treatment plant.

  

Hydrocarbons, C9-C14, aliphatics (2-25% aromatics) have shown to be biodegradable. Therefore, they are not toxic to microorganisms.

 

These data are used as read across data for Hydrocarbons, C12-C16, n-alkanes, isoalkanes, cyclics, 2-25% aromatics.