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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
February 22-24, 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
According to EU Method C.2, with GLP.
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Crustacea, Cladocera
- Source: Daphtoxkit F (Daphnia magna; MicroBioTests Inc., Industriezone "De Prijkels", Venecoweg 19 , 9810 Nazareth, Belgium).
- Age at study initiation (mean and range, SD): less then 24 hours old
- Feeding during test: no

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
250 mg CaCO3/L
Test temperature:
20 ºC throughout the test period
pH:
Between 7.6 and 7.9 in the test substance medium
Dissolved oxygen:
The dissolved oxygen concentration was 9.3-9.5 mg/L in the test substance medium (9.6-9.8 mg/L in control) .
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel:
Disposable multiwell test plates with 30 test wells with a transparent lid, provided with the testkit. Each plate has four
10 mL wells each (replicates A,B,C,D) for the control and for 5 concentrations. Additionally, for each concentration the plates are provided with "rinsing wells" to prevent dilution of the test substance during the transfer of the daphnia from the hatching petri dish to the test wells .
- Aeration: No extra aeration, but slight circulation of air is still possible when the lid of the test plate is closed.

OTHER TEST CONDITIONS
- Photoperiod: artificial light from 4 a.m. to 8 p.m.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilization:
24 and 48 hours after start of exposure to the test substance the neonates were observed for their swimming behavior. Animals not able to swim within approximately 15 s after gentle agitation of the test container are evaluated as immobilized.
Additionally immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities:
- Mortality of control: there was no immobilization in the negative control group
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: the test media were clear and colorless during the whole exposure time

Immobilization of neonates.

The nominal concentration of test substance equivalents is displayed.

The concentrations are presented in units of mg test substance equivalents / L to indicate that not the test substance but a variety of degradation products was tested.

Immobilization 24 hours after the start of exposure

 

T.s.

Immobilised neonates / total number of neonates

Group

concentration

Number per replicate

Total

Total

 

(mg/L)

A

B

C

D

number

%

Control

0

0 / 5

0 / 5

0 / 5

0 / 5

0 / 20

0

T.s.

100

0 / 5

0 / 5

0 / 5

0 / 5

0 / 20

0

Immobilization 48 hours after the start of exposure

 

T.s.

Immobilised neonates / total number of neonates

Group

concentration

Number per replicate

Total

Total

 

(mg/L)

A

B

C

D

number

%

Control

0

0 / 5

0 / 5

0 / 5

0 / 5

0 / 20

0

T.s.

100

0 / 5

0 / 5

0 / 5

0 / 5

0 / 20

0

T.s. test substance

Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 value of the test substance, based on the nominal concentration of test substance equivalents at the start of the assay, was >100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.
Executive summary:

The Acute Toxicity of the test substance to Daphnia magna was determined in a 48 hours static test (According to EU Method C.2). A total of 20 daphnias, divided into 4 replicates (5 daphnias each) were treated with a single dose of 100 mg/L (Limit test). A negative control group, exposed to reconstituted water only, was included. Daphnia immobilization was inspected at 24h and 48 h after the start of the test, as well as immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension. The pH and the dissolved oxygen concentration were tested at the start and at the end of the test. The temperature was controlled at the start, after 24 hours and at the end of the test. Test validity criteria were fulfilled.

The 24 and 48 hours EC50 value of the test substance was > 100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.

Description of key information

The Acute Toxicity of the test substance to Daphnia magna was determined in a 48 hours static test (According to EU Method C.2). The 24 and 48 hours EC50 value of the test substance was > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

A total of 20 daphnias, divided into 4 replicates (5 daphnias each) were treated with a single dose of 100 mg/L (Limit test). Daphnia immobilization was inspected at 24h and 48 h after the start of the test, as well as immobilized animals trapped at the surface were counted to evaluate a possible influence of the test substance to the water surface tension. The temperature was controlled at the start, after 24 hours and at the end of the test.Test validity criteria were fulfilled.

The 24 and 48 hours EC50 value of the test substance was > 100 mg/L. The No Observed Effect Concentration (NOEC) was ≥100 mg/L.