Protein hydrolyzates, silk, reaction products with 3-chloro-2-hydroxypropyl-cocoalkyl-dimethylammonium chloride

Surface tension

Link to relevant study record(s)

Reference

Endpoint:

surface tension

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From November 09, 2017 to January 24, 2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non-GLP and not determined for 90% saturated solution as required by the Guideline

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 115 (Surface Tension of Aqueous Solutions)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.5 (Surface Tension)

Deviations:

no

GLP compliance:

no

Remarks:

The study was conducted in a facility which operates in accordance with Good Laboratory Practice principles; however no claim of GLP compliance was intended nor is made for this study

Type of method:

ring method

Key result

Surface tension:

ca. 41.5 mN/m

Temp.:

20 °C

Conc.:

1 g/L

Results

The readings and temperatures for the sample solutions are shown in the following table:

Table1: Surface tension values

Concentration (mg/L)	Reading (mN/m)	Temperature (°C)
1.003 x 10^4	39.5	19.8
	39.5	19.8
	39.5	19.8
3.009 x 10^3	40.5	19.8
	40.5	19.8
	40.5	19.8
1.003 x 10^3	41.5	19.8
	41.5	19.8
	41.5	19.8
300.9	42.5	19.8
	42.5	19.8
	42.5	19.8
100.3	45.5	19.8
	45.5	19.8
	45.5	19.8
50.15	49.0	19.8
	49.5	19.8
	49.5	19.8
30.09	49.5	19.8
	51.0	19.8
	52.0	19.8
	52.5	19.8
	53.0	19.8
	52.5	19.8
15.04	58.0	19.8
	58.5	19.8
	58.5	19.8
10.03	60.5	19.8
	61.5	19.8
	62.0	19.8
	62.0	19.8
5.015	69.5	19.8
	69.5	19.8
	70.0	19.8
3.009	71.0	19.8
	71.5	19.8
	72.0	19.8
	72.0	19.8
1.003	72.0	19.8
	72.5	19.8
	72.5	19.8
0.301	73.0	19.8
	73.0	19.8
	73.0	19.8

Mean surface tension values were based on the last three readings for a particular concentration which were considered to be constant.

The concentration (mg/L), log₁₀ [concentration (mg/L)] and mean surface tension values for the sample solutions are shown in the following table:

 

Table 2: The concentration (mg/L), log₁₀ [concentration (mg/L)] and mean surface tension values

Concentration (mg/L)	Log10Concentration	Mean Surface Tension (mN/m)	Solution pH
1.003 x 10^4	4.001	39.5	3.98
3.009 x 10^3	3.478	40.5	4.11
1.003 x 10^3	3.001	41.5	4.26
300.9	2.477	42.5	4.48
100.3	2.001	45.5	4.63
50.15	1.700	49.3	5.05
30.09	1.478	52.7	5.24
15.04	1.177	58.3	6.07
10.03	1.001	61.8	5.78
5.015	0.7002	69.7	5.70
3.009	0.4784	71.8	5.70
1.003	0.0013	72.3	5.96
0.301	-0.5216	73.0	5.90

The surface tension at 1 g/L was determined to be 41.5 ± 0.5 mN/m.

Conclusions:

Under the study conditions, the surface tension of 1 g/L nominal test concentration in water was determined to be 41.5 ± 0.5 mN/m at 20 ± 0.5ºC; therefore it is surface-active.

Executive summary:

A study was conducted to determine the surface tension of the test substance using the ring method, according to OECD Guideline 115 and EU Method A.5. Under the study conditions, the surface tension of 1 g/L nominal test concentration in water was determined to be 41.5 ± 0.5 mN/m at 20 ± 0.5ºC. Therefore, the test substance was considered to be surface-active (Envigo, 2018).

Description of key information

Surface tension of the test substance was determined using the ring method, according to OECD Guideline 115 and EU Method A.5 (Envigo, 2018).

Key value for chemical safety assessment

Surface tension:

41.5

in mN/m at 20°C and concentration in mg/L:

1 000

Additional information

(surface active)