Acetic acid, (ethylenedinitrilo)tetra-, cobalt salt

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The study is well-documented by the publication and thus acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Induction of trifluorothymidine-resistant mutants by metal ions in L5178 Y/TK+/- cells

Author:

Amacher, D.E.; Paillet, S.C.

Year:

1980

Bibliographic source:

Mutat. Res. 78, 279-288

Materials and methods

Principles of method if other than guideline:

Induction of TK mutations in mouse lymphoma cells (L5178Y/TK+/- to TK-/-). Treatment was for 3 hrs in the absence of metabolic activation

GLP compliance:

not specified

Type of assay:

in vitro mammalian cell gene mutation tests using the thymidine kinase gene

Test material

Test material information

Constituent 1

Reference substance name:

Cobalt dichloride

EC Number:

231-589-4

EC Name:

Cobalt dichloride

Cas Number:

7646-79-9

Molecular formula:

Cl2Co

IUPAC Name:

cobalt(2+) dichloride

Method

Target gene:

thymidine kinase locus

Species / strain

Species / strain / cell type:

mouse lymphoma L5178Y cells

Details on mammalian cell type (if applicable):

soft-agar media containing RPMI-1640, 10% horse serum, no antibiotics, 1mM sodium pyruvate, 0.02% (w/v) Pluronic F-68 and 0.37% Difco Bacto agar

Additional strain / cell type characteristics:

not specified

Metabolic activation:

without

Test concentrations with justification for top dose:

5.69 - 57.11 µg/ml
6 cultures treated with metal compound and 3 solvent controls

Vehicle / solvent:

saline

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

not specified

Details on test system and experimental conditions:

3 hours at 37°C treatment, following 48 hours at 37°C expression period
incubation for 7 days in TFT containing medium (4µg/mL)

Results and discussion

Test results

Key result

Species / strain:

mouse lymphoma L5178Y cells

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Applicant's summary and conclusion

Conclusions:

The test substance cobalt dichloride hexahydrate did not induce gene mutations in mouse lymphoma cells at the given concentrations and under the conditions applied in the test.