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In a semi-static test with renewal every 24 hours, fish (Danio rerio) were exposed to the substance at nominal concentrations of 0 and 0.5 mg/L (measured 90 -117 % of nominal) during 96 hours. No signs of toxicity and mortality were observed. The LC50 is >0.5 mg/L.

In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L ; measured: 85-103% of nominal) were exposed for 48 hours. Immobility was observed at concentrations of 0.500 mg/L and higher. The EC50 of the substance is 0.492 mg/L (measured).

In a study with a semi-static design (renewal on day 3 and 5) the vegative growth of Lemna gibba G3 was observed for a test period of 7 days by measuring the increase of frond numbers and dry weight. Lemna was exposed at nominal concentrations of 0, 0.00391, 0.0156, 0.0625, 0.250 and 1.00 mg/L (mean measured concentrations 0, 0.000665, 0.00156, 0.0194, 0.138 and 0.660 mg/L). Significant inhibitory effects were determined for yield and growth rate of frond numbers and biomass at test item concentrations of 0.0194 mg/L (measured) and above. The EC50-value for yield of frond numbers was determined to be 0.00385 mg/L (measured), the EC50-value for growth rate of frond numbers was determined to be 0.483 mg/L (measured). The EC50-value for yield of dry weight was determined to be 0.0916 mg/L (measured), the EC50-value for growth rate of dry weight was determined to be 0.220 mg/L (measured).

In a test according to OECD 209, bacteria in sewage sludge were exposed to 6.25, 12.5, 25, 50 and 100 mg/L of the substance during 3 hours under continuous aeration. The inhibition of total respiration was measured over a period of 6 minutes. The EC50 was 36.7 mg/L.

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