4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug 30, 2004 - Nov 17, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

EC Number:

619-232-4

Cas Number:

96624-52-1

Molecular formula:

C36H36

IUPAC Name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Test material form:

solid: crystalline

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
activated sludge, micro organisms from a domestic waste water treatment plant

- Origin
Municipal sewage treatment plant

-Conditioning:
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

According to guideline

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

The biodegradation in % was calculated based on the ThOD of the test item.

Test performance:

The reference item (Aniline) was sufficiently degraded to 60 % after 14 days and to 64% after 28 days of incubation.
The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.

Executive summary:

Purpose

The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The GLP study was performed according to OECD TG 301C.

Result

Biodegradation of test item

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0% as found.

Biodegradation of aniline

The reference item was sufficiently degraded to 60 % after 14 days and to 64 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

Conclusion

The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus the test item is not readily biodegradable.