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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 05 - Jun 17, 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study was performed according to GLP and the methods applied are fully compliant with OECD TG 203.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]
EC Number:
619-232-4
Cas Number:
96624-52-1
Molecular formula:
C36H36
IUPAC Name:
4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]
Test material form:
other: solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
The test medium (reconstituted water and test material) was prepared freshly. Therefore, the calibrated flask with test medium was treated in an ultrasonic device for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the formulation was passed through a membrane filter with a pore size of 0.2 µm. The filtrate was used for the study.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Strain: Dania rerio
- Source: in house
- Length at study initiation: 2.0 ± 1.0 cm
- Weight at study initiation: 8.8 g (test material group), 6.5 g (control group)
- Method of breeding: standard
- Feeding during test: tetra min diet during acclimatization, with daphnia or artemia, or frozen mosquito larva daily


ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Hardness:
250 mg/L CaCO3
Test temperature:
23 -25 °C
pH:
7-8
Dissolved oxygen:
> 60%
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L
- Type (delete if not applicable): open
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12/12 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Clinical Examination: daily
loss of equilibrium, changes in swimming behavior, respiratory function, pigmentation, dead


TEST CONCENTRATIONS
- Spacing factor for test concentrations: -
- Test concentrations: 100 mg/l
- Results used to determine the conditions for the definitive study: pretest
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No aquatic toxicity was observed in this test system up to a nominal concentration of 100 mg/L test material. The 96h LC50 and the 96h NOEC were above the water solubility limit and larger than 100 mg/L (nominal).