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EC number: 619-230-3 | CAS number: 96624-41-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- For this endpoint a one-to-one read across was performed to a chemical similar compound of the same chemical class with a comparable phys. chem. profile and similar response in biological assays. The relevant study was performed according to GLP and the methods applied are fully compliant with OECD TG 201. A detailed read across justification is provided in chapter 13 of this dossier.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 > 100 mg/L (nominal)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 20, 2006 - March 23, 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
The water solubility was below the limit of detection. Therefore the concentration of the test material in water could not be determined and is thus given as nominal value. - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test medium (reconstituted water and test material) was freshly prepared. The calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasound bath for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the preparation was filtered through a filter funnel with a pore size of 10-16 µm. The filtrate was used for the study.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test system used for this study was a green unicellular algae species, Desmodesmus subspicatus, strain No. SAG 86.81, supplied by the "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen". This strain is recommended by international guidelines.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- 250 mg CaCO3
- Test temperature:
- 21-22°C
- pH:
- start of exp.: 7.9+/-0.1
end of exp.: 9.6+/-1.6 - Dissolved oxygen:
- saturated
- Salinity:
- for details see composition of reconstituted water "details on test conditions"
- Nominal and measured concentrations:
- Nominal concentration of 100 mg/L (limit test); due to very low water solubility and the fact that the study was a limit test, no further analysis was initiated.
- Details on test conditions:
- The test medium (reconstituted water and test material) was freshly prepared. The light intensity was approximately 8000 Lux. The experimental part was started by inoculation of algae to the different groups so that the flasks contained about 10,000 cells/mL. The algae were taken from an exponentially growing pre-culture (growth rate 68.8) which was set up 3 days prior to the experimental part under the same conditions as in the final study.
Details on composition of the reconstituted water:
Macro-nutrients:
50.00 mg/L NaHCO3
18.00 mg/L CaCl2 x 2 H2O
15.00 mg/L NH4Cl
15.00 mg/L MgSO4 x 7 H2O
12.00 mg/L MgCl2 x 6 H2O
1.60 mg/L KH2PO4
Trace elements:
100.00 µg/L Na2EDTA x 2 H2O
80.00 µg/L FeCl3 x 6 H2O
415.00 µg/L MnCl2 x 4 H2O
185.00 µg/L H3BO3
7.00 µg/L Na2MoO4 x 2 H2O
3.00 µg/L ZnCl2
1.50 µg/L CoCl2 x 6 H2O
0.01 µg/L CuCl2 x 2 H2O
The pH of the reconstituted water after aeration is approximately 8. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate from separate study
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- Regularly, a positive control test to check the test system is carried out with potassium dichromate (no results provided in the study report).
- Validity criteria fulfilled:
- yes
- Conclusions:
- An aqueous preparation of 100 mg/L (nominal) of the test material revealed no toxic effect in the test system. Analytically, the 72 hours EC50 to Desmodesmus subspicatus could not be determined; it exceeded the maximum solubility of the test material in reconstituted water (< 0.06 mg/L).
Referenceopen allclose all
Description of key information
This one-to-one read across uses data generated for a chemical similar compound. For this compound information is available from a study that was performed according to GLP and to OECD TG 201 study, giving a NOEC of > 100 mg/L (nominal). Thus no toxicological effect occurs up to the limit of water solubility.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
For this endpoint information from a GLP compliant OECD TG 201 study is available. The data were obtained for a chemical similar compound and used in a one-to-one read across.
Justification for read across
Type of read across:one-to-one
Based on:
(1) chemical similarity: both compounds share a bicyclohexyl core structure and differ in alkyl substitution pattern
(2) almost identical physicochemical profile
(3) same response in relevant biological assays
Assay |
Test item |
surrogate |
Appearance |
solid |
solid |
Similarity Score (Dice) |
1,0 |
1,0 |
Water solubility |
< 0,004 mg/L |
<0,06 mg/L |
logP |
8,2 |
4,4 |
Molar mass |
236,4 g/mol |
264,5 g/mol |
Melting point |
65,7 °C |
97,1 °C |
Boiling Point |
323,7 °C |
352 °C |
Vapour Pressure |
0,024 Pa |
0,0041 Pa |
Fish acute (OECD 203) | NOEC > 100 mg/L (nominal) | NOEC > 100 mg/L (nominal) |
Fish long term data (from OECD 305) | NOEC > 100 mg/L (nominal) | NOEC > 100 mg/L (nominal) |
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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