2-butyl-4-chloro-4,5-dihydro-5-hydroxymethyl-1-[2'-(2-triphenylmethyl-1,2,3,4-2H-tetrazol-5-yl)-1,1'-biphenyl-4-methyl]-1H-imidazole

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

Bacteriologcal test (Suspension of bacterial cells) with metabolic activation / without metabolic activation

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-butyl-4-chloro-4,5-dihydro-5-hydroxymethyl-1-[2'-(2-triphenylmethyl-1,2,3,4-2H-tetrazol-5-yl)-1,1'-biphenyl-4-methyl]-1H-imidazole

EC Number:

412-420-5

EC Name:

2-butyl-4-chloro-4,5-dihydro-5-hydroxymethyl-1-[2'-(2-triphenylmethyl-1,2,3,4-2H-tetrazol-5-yl)-1,1'-biphenyl-4-methyl]-1H-imidazole

Cas Number:

133909-99-6

Molecular formula:

C41H37ClN6O

IUPAC Name:

[2-butyl-4-chloro-1-({2'-[2-(triphenylmethyl)-2H-1,2,3,4-tetrazol-5-yl]-[1,1'-biphenyl]-4-yl}methyl)-1H-imidazol-5-yl]methanol

Method

Target gene:

Bacterial cells

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Supplemented liver fraction (S-9 mix) prepared from an animal previously treated with Aroclor 1254

Test concentrations with justification for top dose:

Concentration range in the main test (µg/plate)
with metabolic activation : 50 5000
without metabolic activation : 50 5000
Concentration of test substance observed to be toxic to bacteria (µg/plate) :
a) in a preliminary test with metabolic activation >5000
without metabolic activation > 5000
b) in the main test with metabolic activation >5000
without metabolic activation > 5000
Concentration of the test substance resulting in precipitation : 1500 µg/plate

Controls

Untreated negative controls:

yes

Remarks:

Dimethyl sulfoxide

Negative solvent / vehicle controls:

yes

Remarks:

Dimethyl sulfoxide

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

With metabolic activation : negative

Without metabolic activation : negative