Phenol, 4,4'-(1-methylethylidene)bis-, oligomer with (chloromethyl)oxirane and ethylenediamine, reaction products with carbon disulphide, potassium salts

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

72 hours. Performed in 2004

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

Marine study on invertebrates following guidance. No confirmatory analysis, but substance is soluble and stable in water.
The product testeed was commercial grade and included solvents; these solvents are of low toxicity to aquaitc and marine organisms and are not considered to have affected the outcome of the results.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

Using marine species

GLP compliance:

yes

Specific details on test material used for the study:

Test material supplied to the laboratory as ca 50% commercial grade mixture in ethanediol and butanol with water.
The presence of these solvents were not considered to impact on the test result.

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Skeletonema costatum

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

Guideline

Test temperature:

20 C

pH:

8.44

Salinity:

Seawater, ca 3.3% salinity

Nominal and measured concentrations:

Nominal 0, 0.1,.05, 2.5 and 10 mg/l based on material received by the laboratory
Estimated 0, 0.05, 0.25, 1.25 and 5 mg/l actives based on 50% solution used

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol at 1.5 mg/l

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

ca. 6.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

ca. 3.1 mg/L

Nominal / measured:

estimated

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Details on results:

The IC50 was estimated to be ca 3.1 mg/l based on the active ingredient
The presence of solvents in the commercial grade material tested are not thought to have impctedon the results.

Validity criteria fulfilled:

yes

Conclusions:

The IC50 was estimated to be ca 3.1 mg/l based on the active ingredient
The presence of solvents in the commercial grade material tested are not thought to have impctedon the results.

Description of key information

The IC50 was estimated to be ca 3.1 mg/l based on the active ingredient

The presence of solvents in the commercial grade material tested are not thought to have impacted on the results.

Key value for chemical safety assessment

EC50 for marine water algae:

3.1 mg/L