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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
For this endpoint information from structural similar compounds (CAS 132123-39-8) is available. The studies for these similar compounds were performed according to GLP and the methods applied are fully compliant with OECD TG 301. See chapter 13 report for a more detailed justification.
Reason / purpose for cross-reference:
read-across source
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 April - 02 July, 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant
- OriginMunicipal sewage treatment plant
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice.
- Concentration of suspended solids per litre: 30 mg
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
According to guideline
Reference substance:
aniline
Test performance:
The reference item (Aniline) was sufficiently degraded to 77 % after 14 days and to 74% after 28 days of incubation.The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Key result
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
28 d
Results with reference substance:
14d: 77 %, 28d: 74 %
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Executive summary:

The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The substance concentration during the course of this study was monitored by LC with UV detection (254 nm). As a reference item aniline was tested simultaneously under the same conditions as the test item and functioned as a procedure control. The GLP study was performed according to OECD TG 301C.

Result

Biodegradation of test item

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0 % as found. HPLC analysis confirmed that no degradation occurs.

Biodegradation of aniline

The reference item was sufficiently degraded to 77 % after 14 days and to 74 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

Conclusion

The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus, the test item is not readily biodegradable.

Description of key information

OECD 301F (Read Across): not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information