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EC number: 923-927-0 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 days
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Lot No:1030601011
Purity: 100%
Appearance: White solid
Water solubility: Approx 60g/100 ml at 20°C
Supplier: Media Services Ltd - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Acitvated Sludge:
1) Mixed Liqor suspended solid (MLSS): 3250 mg/L
2) Source: Chemical Evaluation and Research Institute, Japan
3) Date of receipt: jan 15, 2004 - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 25 ± 1 °C
TEST SYSTEM
Closed system oxygen consumption measuring apparatus, Ohkura Electric Co, Model OM-2001
- Reference substance:
- aniline
- Parameter:
- % degradation (DOC removal)
- Value:
- 101
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 90
- Sampling time:
- 28 d
- Parameter:
- % degradation (test mat. analysis)
- Value:
- > 99
- Sampling time:
- 28 d
- Details on results:
- Due to sampling times, it was difficult to assess if 10-day window was met, but by Day 14, in excess of 60% degradation had been observed.
- Results with reference substance:
- The degradability of aniline, based on BOD, was 73% after 7 days. The activity of the sludge was thus shown to be satisfactory.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance was considered to be readily biodegradable based on the BOD, DOC and test substance concentration measured in the test.
- Executive summary:
The test substance, VERTEC SPC was tested for ready biodegradablity in the OECD 301 C test. Based on the BOD, DOC and test substance concentration measured in the test, the test substance was considered to be readily biodegradable.
Reference
Degradability based on BOD
Bottle No |
Test Substance |
ThOD mg |
Day 7 |
Day 14 |
Day 21 |
Day 28 |
||||
BOD |
% Deg |
BOD |
% Deg |
BOD |
% Deg |
BOD |
% Deg |
|||
1 |
Aniline |
90.2 |
66.0 |
73 |
71.2 |
79 |
73.6 |
82 |
73.7 |
82 |
2 |
-- |
-- |
0.0 |
-- |
0.0 |
-- |
0.0 |
-- |
0.0 |
-- |
3 |
Vertec STC |
18.0 |
10.0 |
56 |
13.5 |
75 |
16.1 |
89 |
16.2 |
90 |
4 |
Vertec STC |
18.0 |
10.0 |
56 |
13.5 |
75 |
16.1 |
89 |
16.2 |
90 |
5 |
Vertec STC |
18.0 |
10.0 |
56 |
13.5 |
75 |
16.1 |
89 |
16.2 |
90 |
6 |
Vertec STC |
18.0 |
0.0 |
-- |
0.0 |
-- |
0.0 |
-- |
0.0 |
-- |
PH
Bottle No |
pH |
|
Day 0 |
Day 28 |
|
1 |
-- |
7.9 |
2 |
6.9 |
7.1 |
3 |
7.0 |
8.4 |
4 |
7.2 |
8.6 |
5 |
7.0 |
8.5 |
6 |
3.9 |
3.8 |
Degradability based on DOC
Bottle No |
Organic carbon added, mg/L |
DOC mg/L |
% Degradability |
2 |
-- |
1.3 |
-- |
3 |
28.6 |
1.1 |
101 |
4 |
28.6 |
1.1 |
101 |
5 |
28.6 |
1.0 |
101 |
6 |
28.6 |
29.1 |
-- |
Degradability based on residual test substance
Bottle No |
Concentration mg/L |
% Degradability |
2 |
<1 |
-- |
3 |
<1 |
>99 |
4 |
<1 |
>99 |
5 |
<1 |
>99 |
6 |
95.0 |
-- |
Description of key information
Test performed on titanium citrate sodium salt; this demonstrates biodegradation of citrate salts.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
[Type of water: freshwater]
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