Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 hours
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Publication describing toxicity of citric acid to different organisms
Peer reviewed

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)
Principles of method if other than guideline:
Toxicity threshold testing with soil dwelling bacteria Pseudomonas putida
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Citric acid
EC Number:
201-069-1
EC Name:
Citric acid
Cas Number:
77-92-9
Molecular formula:
C6H8O7
IUPAC Name:
citric acid
Test material form:
not specified

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Remarks:
Water

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Taken from laboratory stock

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
20 C

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
IC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
Material was non-toxict to limit of testing

Applicant's summary and conclusion

Conclusions:
Citric acid and citrates in general occur in living cells and is not considered toxic to mibrocial organisms.
Titanium salts are poorly soluble under dilute conditions in natural waters. There are no reports of toxicity from titanium salts.