3-(isodecyloxy)propylammonium acetate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 d

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Result of Assay (Purity): 93.7%
- Storage conditions: Ambient (21°C to 29°C)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source: The source of test organisms was secondary effluent freshly obtained from a municipal sewage treatment plant: receiving predominantly domestic sewage from the Sewage Treatment plant, Chandana Complex, Tumkur -572103.
- Treatment: Secondary effluent was filtered through a coarse filter paper; the first 200 mL was discarded. The filtrate was kept aerated up to inoculation.
- Inoculation: A volume of 5 mL filtrate of secondary effluent per L of final volume.

Duration of test (contact time):

28 d

Initial conc.:

ca. 2.44 other: mg of O2/mg

Based on:

ThOD

Details on study design:

TEST CONDITIONS
- The test vessels were held at at 22 ± 2 °C in dark conditions for the duration of the study.
- Illumination: The test bottles were protected from light.
- Preparation of Mineral medium:
Solution A: Weighed accurately 0.8859 g of Potassium dihydrogen orthophosphate (KH2PO4), 2.1800 g of Dipotassium hydrogen orthophosphate (K2HPO4), 3.3568 g of Disodium hydrogen orthophosphate dehydrate (Na2HPO4.2H2O) and 0.0516 g of Ammonium Chloride (NH4Cl) were dissolved in Milli-Q water and made up to 100 mL with the same. The pH of the solution was adjusted to 7.51.
Solution B: Accurately weighed 2.7601 g of Calcium Chloride dihydrate (CaCl2.2H2O) was dissolved in Milli-Q water and made up to 100 mL with the same.
Solution C: Weighed accurately 2.2559 g of Magnesium sulphate heptahydrate (MgSO4.7H2O) was dissolved in Milli-Q water and made up to 100 mL with the same.
Solution D: Weighed accurately 0.0252 g of Iron (III) chloride hexahydrate was dissolved in Milli-Q water (FeCl3.6H2O) and made up to 100 mL with the same.

Reference substance:

acetic acid, sodium salt

Preliminary study:

Preparation of Mineral Medium:The medium was prepared by mixing 25 mL of solution A with 20000 mL of Milli-Q water and 25 mL of B, C and D were made up to 25 L with Milli-Q water. Strongly aerate mineral medium for at least 20 minutes and allow standing. Generally, the medium is ready for use after standing for 20 hour at the test temperature. Carry out each test series with mineral medium derived from the same batch. The concentration of dissolved oxygen for control purposes, the value wa s 8.10 mg/L at 22.2 °C.

- Preparation of Test Item Solutions: Accurately 0.1035 g and 0.1016 g of test item was weighed in to 100 mL standard measuring flask separately, and diluted with Milli-Q water and made up to the mark using Milli-Q water. The test item was completely soluble in Milli-Q water and the solubility of test item was found to be 1g/L. Accurately weighed 250.05 mg of test item in to beaker and quantitatively transferred in to 1000 mL volumetric flask and diluted with Milli-Q water to dissolve the contents and the volume was made up to the mark with Milli-Q water.

- Preparation of Reference Substance Solutions: Accurately 500.10 mg of reference substance of sodium acetate was weighed in to 1000 mL standard measuring flask containing 800 mL of Milli-Q water and made up to the mark using Milli-Q water.

Test performance:

Individual BOD bottles were be prepared for each measuring point, except that the bottle measured at the start and also measured on day 7. These parallel groups were prepared to allow duplicate measurements of oxygen consumption at the test intervals. 10 day window was also determined.

Key result

Parameter:

% degradation (DOC removal)

Value:

>= 60 - <= 79.18

Sampling time:

28 d

Remarks on result:

other: readily biodegradable

Details on results:

During the study, the determination of oxygen concentrations were performed at the start of the experiment (day 0) and on 7, 10, 14, 21 and 28 days.
All differences of duplicate biodegradation values expressed as mg O2/L were less than 20% (-1.18%).
After 28 days oxygen depletion in the inoculum blank obtained was 0.98 mg O2/L which is below 1.5 mg O2/L.
The residual concentrations of oxygen in the test bottles were found to be > 0.5 mg/L at all durations.
The total nitrogen (N) was determined at all the pre-determined intervals and observed there was no significant increase in Oxidized Nitrogen from the start to end of the experiment.

Key result

Parameter:

ThOD

Value:

>= 60 - <= 65.62 other: mg of O2/mg of test mat.

Results with reference substance:

The test substance should reach 60% of degradation (% of ThOD) by 28 day. The pass level for ready biodegradability is 60% degradation. This pass value should reach in a 10 day window within a 28 days period. The 10 day window begins when the degree of biodegradation should reach 10% and should end before 28 day of the test.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item (3-(Isodecyloxy) Propylammonium Acetate) is readily biodegradable under the conditions of the closed bottle test according to the OECD guideline 301 D.

Executive summary:

The Ready biodegradability of the3-(Isodecyloxy) Propylammonium Acetatewas determined using Closed bottle test as perOECD guideline No. 301 D, 1992.

Test item was ayellowish liquid with apurity of about 93.7 % and completely soluble at the concentrations tested. The ThOD of test item3-(Isodecyloxy) Propylammonium Acetate)was calculated to be 2.44 mg of O₂/mg. For the calculation of the ThOD the information of the main component of the test item (3-(Isodecyloxy) Propylammonium Acetate) was used. The ThOD of sodium acetate (reference substance) was calculated to be 0.78 mg of O₂/mg. The values for the test and reference substances were determined using the molecular formula.

During the study, the determination of oxygen concentrations were performed at the start of the experiment (day 0) and on day 7, 10, 14, 21 and 28.

The test item reached a maximum biodegradability of 79.18% based on ThOD within 28 days of biodegradation exceeding 60%.

All differences of duplicate biodegradation values expressed as mg O₂/L were less than 20% (-1.18%).

After 28 days oxygen depletion in the inoculum blank obtained was 0.98 mg O₂/L which is below 1.5 mg O₂/L.

The residual concentrations of oxygen in the test item bottles were found to be > 0.5 mg/L at all durations.

 

The reference substance (sodium acetate) attained a 65.62% biodegradation level                  (greater than 60% degradation) within 14 days based on ThOD.

 

The percentage degradation of the toxicity control has attained a maximum of 76.61%(greater than 25% degradation) by 14 days. Therefore thetest item was concluded to be non- inhibitory to the microbial inoculum.

 

The total nitrogen (N) was determined at all the pre-determined intervals and observed there was no significant increase in Oxidized Nitrogen from the start to end of the experiment.

 

Since all criteria for acceptability of the test were met, this study was considered to be valid.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Duration of test (contact time):

28 d

Details on study design:

TEST CONDITIONS
- Composition of medium:
The following stock solutions were prepared.

Solution A : About 8.50 g of Potassium dihydrogen orthophosphate (KH2PO4), 21.75 g of Dipotassium hydrogen orthophosphate (K2HPO4), 33.40 g of Disodium hydrogen orthophosphate dehydrate (Na2HPO4.2H2O) and 0.50 g of Ammonium Chloride (NH4Cl) will be dissolved in water and make up to 1 L. The pH should be 7.4 (±0.2). If necessary, the pH will be adjusted with NaOH or HCl.

Solution B : About 27.50 g of Calcium chloride anhydrous, (CaCl2) or 36.40 g of Calcium chloride dihydrate (CaCl2.2H2O) will be dissolved in water and make up to 1 L.
Solution C : About 22.50 g of Magnesium sulphate heptahydrate (MgSO4.7H2O) will be dissolved in water and make up to 1 L.

Solution D : About 0.25 g of Iron (III) chloride hexahydrate (FeCl3.6H2O) will be dissolved in water and make up to 1 L.
Note: In order to avoid having to prepare this solution immediately before use, one drop of concentrated HCl or 0.4 g Ethylene-diaminetetra-acetic acid (EDTA disodium salt) per L will be added.
The medium will be prepared by mixing 1 mL of solution (A) with 800 mL of water and 1 mL of solutions B, C, D and made up to 1 L with water.

- Test temperature: 22 C
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water: yes, 20 minutes

- Continuous darkness: yes

Reference substance:

acetic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 79.18

Sampling time:

28 d

Interpretation of results:

readily biodegradable

Description of key information

During the study, the determination of oxygen concentrations were performed at the start of the experiment (day 0) and on day 7, 10, 14, 21 and 28. The test item reached a maximum biodegradability of 79.18% based on ThOD within 28 days of biodegradation exceeding 60%.

The test item (3-(Isodecyloxy) Propylammonium Acetate) is readily biodegradable under the conditions of the closed bottle test according to OECD criteria.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater