4,4-dimethyloxazolidine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

9 - 11 November 1979

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Standard Methods for the Examination of Water and Wastewater

Version / remarks:

American Public Health Association, 14th ed., 1975

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians

Version / remarks:

U.S. EPA, Ecol. Res. Ser. 660/3-75009, 1975

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4-dimethyloxazolidine

EC Number:

257-048-2

EC Name:

4,4-dimethyloxazolidine

Cas Number:

51200-87-4

Molecular formula:

C5H11NO

IUPAC Name:

4,4-dimethyl-1,3-oxazolidine

Test material form:

liquid

Specific details on test material used for the study:

Test material name: Amine CS-1135
Lot No.: 94
Purity: Oxadine: 82.01% (same test material as daphnia study)
Appearance: clear liquid
Storage conditions: 1°C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

- 250 mL glass beakers containing 200 mL of ABC well water.
- Five concentrations (10 mg/L, 18 mg/L, 32 mg/L, 56 mg/L, 100 mg/L) in duplicate, with ten Daphnia each

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house culture at the ABC facilities
- Age: adults, < 24 hours old
- Feeding: Daphnia were fed a suspension of trout chow and alfalfa (PR-11) daily until 24 hours prior testing

Study design

Test type:

static

Water media type:

other: ABC well water

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

255 ppm CaCO3

Test temperature:

20°C +-1°C

pH:

8.2

Dissolved oxygen:

9.3 ppm

Salinity:

not reported

Conductivity:

50 µmhos/cm

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass flask containing 200 mL of test medium, two flasks per dosage level, placed on a bench top in a controlled temperature area
- Number of Daphnia: 10 Daphnia per vessel
- The photoperiod was controlled to give 16 hours daylight and 8 hours nightfall

RANGE-FINDING STUDY
Prior to the definitive test a range finding test was conducted using 10 Daphnia per dossage level.

EFFECT PARAMETERS MEASURED
Daphnia were observed for mortality and/or adverse behavioral effects at 24 and 48 hours after compound administration.
Temperature, dissolved oxygen and pH is monitored throughout the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

45 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mortality

Remarks on result:

other: Statistical result using a computerized program (moving average test)

Details on results:

The estimated LC50 at 24 hr was 75 mg/L.
The 48 hr "no effect" level was 32 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The acute toxicity of the test material to Daphnia magna was assessed using the methods outlined by the Commitee on Methods for Toxicity Tests with Aquatic Organisms (US methods). Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits. The estimated LC50 at 24 hr was 75 (56-100) mg/L and at 48 hr was 45 (32-56) mg/L (95 percent confidence limits in parenthesis). The 48 hr "no effect" level was 32 mg/L.