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EC number: 295-374-7
CAS number: 92044-94-5
Tab. 6: EC50 Values of Biomass Inhibition
and Rate-Related Inhibition of the Reference item based on nominal
concentrations mg/L, (0-72 h)
Inhibition of biomass growth[mg/L]
95% confidence interval
0.42 - 0.55
Rate-related inhibition [mg/l]
0.69 - 1.04
The acute toxicity of
number 6480) to the unicellular freshwater
green alga Scenedesmus subspicatus was
determined according to the principles of
OECD- Guideline 201, at
DR.U.NOACK-lABORATORIUM FÜR ANGEWANDTE
BIOLOGIE in 31157 Sarstedt, Germany from
June 12 to 15, 2001.
The study was conducted under
static conditions over a duration of 72
hours with an initial cell density of
nominally 104 cells/ml. A saturated solution
served as stock solution and further 4
dilution levels in a geometrical series with
a dilution factor 2 were prepared from this
solution as listed in the following
table.Corresponding DOC concentrations are
Test item concentrations
Dilution of stock solution
3 replicates were tested for each concentration level and
6 replicates for control.
The saturated solution and all dilution levels were found
to be clear throughout the test.
Microscopic evaluation of the cells at the start of the
incubation period revealed no morphological abnormalities. At the end
cells at concentration levels of 0.3 to 2.5 mg DOC/L were larger as in
Water quality parameters of pH-value, measured at 0 and
72 h, and room temperature, measured continuously, were determined to be
within the acceptable limits.
The effects of Wollwachsalkohol/lanolinalkohol, based on
the initially measured DOC concentrations are summarized in the
of Biomass Inhibition and Rate-Related Inhibition based on the initially
measured DOC [mg/L] of
the saturated solution, (0-72 h) NOEC calculated using one way analysis
of variance andDUNNETT'Stest.
Inhibition of biomass growth [mgDOC/L]
95% Confidence interval
Rate-related inhibition [mgDOC/L]
In this study Wollwachsalkohol/lanolinalkohol caused only
minor effects on the freshwater green alga Scenedesmus subspicatus when
tested up to the saturated aqueous solution. No dose-effect-relationship
was observed. The EC50-values for inhibition of biomass growth (EbC50
) and specific growth rate (ErC50 ) after 72 h
were > 2.5 mg DOC/L for both end points.
A study was performed to assess the effect
of the test item on the growth of the green
alga Desmodesmus subspicatus. The method
followed that described in the OECD
Guidelines for Testing of Chemicals (2006)
No 201, "Freshwater Alga and Cyanobacteria,
Growth Inhibition Test" referenced as Method
C.3 of Commission Regulation (EC) 440/2008.
Following a preliminary range-finding test,
Desmodesmus subspicatus was exposed to a
Water Accommodated Fraction (WAF) of the
test item, at a single nominal loading rate
of 100 mg/l (six replicate flasks) for 72
hours, under constant illumination and
shaking at a temperature of 24 ± 1 °C.
Samples of the algal
populations were removed daily and cell
concentrations determined for each control
and treatment group, using a Coulter®
Multisizer Particle Counter.
Exposure of Desmodesmus subspicatus to the
test item gave EL*50 values of greater than
100 mg/l loading rate WAF and
correspondingly the No Observed Effect
Loading Rate was 100 mg/l loading rate WAF.
It was considered unnecessary
and unrealistic to test at loading rates in
excess of 100 mg/l.
Total Organic Carbon (TOC)
analysis of the test preparations was
performed at 0 and 72 hours. Given the
background level of carbon in the control
vessels and also the low level of carbon in
the test vessels, it was considered that the
results gave no evidence of the presence of
test item in the WAF.
Therefore, given that the
toxicity cannot be attributed to a single
component or a mixture of components but to
the test item as a whole, and the dissolved
test item was below the quantifiable limit
of the analytical method, the results were
based on nominal loading rates only.
* EL =Effective Loading Rate
The acute toxicity of Lanolin alcohol to the unicellular freshwater
green alga Scenedesmus subspicatus was determined under static
conditions over a duration of 72 hours. Exposure concentrations were
0.15, 0.3, 0.6, 1.3 and 2.5 mg/L (as DOC/L) formulated
based on a filtered stock solution at 1000 mg/L. No
dose-effect-relationship was observed. The EC50-values for inhibition of
biomass growth (EbC50 ) and specific growth rate (ErC50) after 72 h
were> 2.5 mg DOC/L for both end points (Scheerbaum 2001).
A study according to OECD 201 was performed to assess the effect of the
Lanolin fatty acids on the growth of the green alga Desmodesmus
subspicatus. The algae were exposed to a WAF at 100 mg/L loading rate
during 72 hours. EL50 values were greater than 100 mg/l loading rate WAF. Correspondingly
the No Observed Effect Loading Rate was 100 mg/l loading rate WAF
The ester of lanolin alcohols and fatty acids is expected to be
hydrolysed enzymatically and has been shown to hydrolyse abiotically.
Therefore the toxicity of the hydrolysis products lanolin alcohols and
lanolin fatty esters is considered representative for the toxicity of
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