Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Jul - 16 Dec 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1981

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPP 81-1 (Acute Oral Toxicity)

Version / remarks:

1978

Deviations:

no

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

EC Number:

220-482-8

EC Name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Cas Number:

2781-11-5

Molecular formula:

C9H22NO5P

IUPAC Name:

diethyl {[bis(2-hydroxyethyl)amino]methyl}phosphonate

Test material form:

liquid: viscous

Details on test material:

- Physical state: viscous liquid
- Colour: amber
- Expiry date: not reported

Specific details on test material used for the study:

- Receipt date: 25 Jun 1981
- Color: amber
- Form: viscous liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratories, Portage, USA
- Weight at study initiation: 152-185 g (females), 176-212 g (males)
- Fasting period before study: 16-18 hours prior to treatment

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Amount of vehicle: 10 mL/kg bw

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

10 males and females per dose group and per control

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: minimally twice a day; only once a day on holidays and weekends
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occurred during the study period.

Clinical signs:

other: other: Females: piloerection and red facial stains in all animals; one rat with alopecia Males: mild depression and red facial stains

Gross pathology:

Females: reddened intestines
Males: no adverse effects

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the test conditions, the test material is not classified according to Regulation (EC) No. 1272/2008 (CLP) and to the GHS.

Executive summary:

In an acute toxicity study conducted according to the OECD TG 401, Sprague-Dawley rats (5/sex) were administed with the test material diluted in corn oil at a single oral (gavage) dose of 5000 mg/kg bw.

 

No mortality occurred during the study period.

Piloerection and red facial stains were observed in in all females. One female also had alopecia. Mild depression and red facial stains were noted in males.

 

Combined Oral LD50 > 5000 mg/kg bw.

 

Under the test conditions, the test material is not classified according to Regulation (EC) No. 1272/2008 (CLP) and to the GHS.