Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

old study report with limited documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

2074

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

1981

GLP compliance:

no

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

EC Number:

220-482-8

EC Name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Cas Number:

2781-11-5

Molecular formula:

C9H22NO5P

IUPAC Name:

diethyl {[bis(2-hydroxyethyl)amino]methyl}phosphonate

Test material form:

liquid

Specific details on test material used for the study:

- Color: yellow-brown
- Form: liquid
- Relative density: 1.164 (at 20°C)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Breeder Winkelmann, Borchen, Germany
- Weight at study initiation: 160-180 g
- Housing: 5 animals of same sex per cage in makrolon cages (type III)
- Diet: "Altromin-R-Standardkost" ad libitum
- Water: tap water ad libitum

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- System of generating aerosols: The test substance was mixed with a solution of alcohol-Lutrol (1:1) containing oil red and sprayed in the inhalation chambers.

TEST ATMOSPHERE
- Brief description of analytical method used: Spray mist from the inhalation chamber was absorbed with cotton wool and oil red was extracted with Xylol. The concentration of the test substance in the air was indirectly measured by the extinction of the red coloured solution at 525 nm (the amount of oil red/L air equals the amount of test substance).
- Samples taken from breathing zone: yes

Duration of exposure:

>= 1 - <= 4 h

Remarks on duration:

Exposure for 1 or 4 h

Concentrations:

524 mg/m^3 for 1 h exposure
243 and 520 mg/m^3 for 4 h exposure

No. of animals per sex per dose:

10 males and 10 females per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: no

Statistics:

LC50 was calculated via probit analysis

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 524 mg/m³ air

Based on:

test mat.

Exp. duration:

1 h

Effect levels 2

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 520 mg/m³ air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

No mortality occurred during the study period.

Clinical signs:

other: No clinical signs were observed during the study period.

Body weight:

Body weight was not examined during the study period.

Gross pathology:

Gross pathology was not performed.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the test conditions, the test material is not classified according to Regulation (EC) No. 1272/2008 (CLP) and to the GHS.

Executive summary:

In an acute inhalation toxicity study conducted similarly to the OECD TG 403, groups of Wistar rats (10/sex) were exposed by inhalation route to the test material mixed with a solution of alcohol-Lutrol (1:1) for 1 or 4 hours to whole body at concentrations of 524 mg/m3 (1-hour exposure), 243 or 520 mg/m3 (4-hour exposure). Animals were then observed for 7 days.

 

No mortality occurred during the study period. No clinical signs were observed.

 

Combined Inhalation LC50 > 524 mg/m3 (1-hour exposure).

Combined Inhalation LC50 > 520 mg/m3 (4-hour exposure).

 

Under the test conditions, the test material is not classified according to Regulation (EC) No. 1272/2008 (CLP) and to the GHS.