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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: National Guideline with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Guideline issued by Ministry of Agriculture, Fisheries and Food, United Kingdom, AEP2, 1984
Principles of method if other than guideline:
96-h exposure of marine species Crangon crangon to 5 concentrations of the test substance under semi-static conditions. Mortality is recorded.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopropyl oleate
EC Number:
203-935-4
EC Name:
Isopropyl oleate
Cas Number:
112-11-8
Molecular formula:
C21H40O2
IUPAC Name:
isopropyl octadec-9-enoate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since the test material was immiscible with water, constant stirring of test solution was applied to ensure a reasonable amount of test substance remained in suspension. The tests animals where shielded from the stirrer.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes, a floating layer above the test solution

Test organisms

Test organisms (species):
Crangon crangon
Details on test organisms:
TEST ORGANISM
- Common name: Brown shrimp
- Source: wild cought by Mr. R.J. Pipe in Burnham-on-Crouch, Essex; specimen collected in lower estuary, River Crouch, on 27th Feb 1991
- Weight at study initiation: mean weight 1.072 g ± 0.221 g
- Length at study initiation: mean length 50.9 mm ± 3.285 mm
- Feeding during test: no

ACCLIMATION/HOLDING
- Acclimation conditions: at least two days holding prior the test in artificial seawater (Marinemix), formulated by Wiegandt GmbH in the same temperature range as in main test
- feeding during acclimation: minced ox heart (approx. 1% of body weight per day)
- no further data on conditions and observations during acclimation are provided

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
14.48 °C (mean)
pH:
7.8 - 8.4
Dissolved oxygen:
83 - 89% (air saturation value)
Nominal and measured concentrations:
nominal: 850, 1530, 2640, 4760 and 8500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L glass aquaria
- Aeration: constant; air continuously diffused through the aquaria
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Artificial seawater (Marinemix), formulated by Wiegandt GmbH

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: mortality after 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Range finding study: was conducted, no details given
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 8 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- No mortality observed at all concentrations (0 - 8500 mg/L) during the test period of 96 hours.
- Part of the test sample was observed to remain on the liquid surface, even with continuous stirring. The agitation was sufficient to maintain a distribution within the body of the liquid, but at higher concentrations the thickness of the floating layer increased. Thus concentrations above 1% v/v were not evaluated.

Applicant's summary and conclusion