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EC number: 205-741-5 | CAS number: 149-45-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 May - 14 Jun 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
Test material
- Reference substance name:
- Disodium 4,5-dihydroxybenzene-1,3-disulphonate
- EC Number:
- 205-741-5
- EC Name:
- Disodium 4,5-dihydroxybenzene-1,3-disulphonate
- Cas Number:
- 149-45-1
- Molecular formula:
- C6H6O8S2.2Na
- IUPAC Name:
- disodium 4,5-dihydroxybenzene-1,3-disulphonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and 100 mg/L
- Sampling method: Samples were taken at test start and test end
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Differential loading: A stock solution containing 101 mg/L test item in dilution water was prepared and used for the test.
- Controls: Yes, only medium
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Berlin
- Source: Originally obtained from Bundesumweltamt Berlin, in-house breading since 27. Sep 2007
- Feeding during test: No
ACCLIMATION
- Acclimation conditions: Medium was different M4-medium (husbandry) to dilution water (test)
- Type and amount of food: Green algae (Desmodesmus subspicatus) for stock culture
- Feeding frequency: Twice a week
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: 20 h before test start, the adult animals were separated from the young. 40 min before test start, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 19 h and 20 min, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/L and 250 mg CaCO3/L (for dilution water)
- Test temperature:
- 20.3 – 21.8 °C
- pH:
- 7.8 (Control)
7.2 - 7.8 (100 mg/L) - Dissolved oxygen:
- 8.7 - 8.8 mg/L (Control)
8.7 - 8.8 mg/L (100 mg/L) - Nominal and measured concentrations:
- Control and 100 mg/L (nominal)
Control, 119.1 mg/L (based on geomertic mean of each test item concentration) - Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open (covered with glas lids)
- Material, size, headspace, fill volume: glass beakers, nominal volume 50 mL, tall shape, 20 ± 5 mL test solution
- Aeration: No
- No. of organisms per vessel: 5 organisms
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, details are given in table 1 in section "any other information on materials and methods incl. tables".
- Culture medium different from test medium: Yes M4-medium (for husbandry) and dilution water (for the test)
OTHER TEST CONDITIONS
- Adjustment of pH: No
EFFECT PARAMETERS MEASURED: Mobility of Daphnia was checked each day
RANGE-FINDING STUDY: No range-finding test was performed - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 119.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No abnormal behaviour was observed
- Mortality of control: No mortality was observed in the control replicates
- Other: Results are summarized within table 1 in section"Any other information on results incl tables" - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study. The 24h-EC50 value was determined as 1.9 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as specified in the OECD guideline. Since the fits showed sufficient statistical correspondence of the data with the dose-response-equation, this reference study can be considered valid.
- Reported statistics and error estimates:
- As no toxicity occurred, no statistical evaluation was conducted.
Any other information on results incl. tables
Analytical Results:
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV-determination. The concentration determined at the start of the test was 116% of the nominal concentration. At the end of the test the determined concentration was 122% of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentration. However, nominal concentrations were additionally presented.
Table 1: Biological Results Test Item
Parameter |
Nominal value |
Geometric mean value |
48h-NOEC |
≥ 100mg/L |
≥ 119.1 mg/L |
48h-LOEC |
> 100 mg/L |
> 119.1 mg/L |
24h-EC50 |
> 100 mg/L |
> 119.1 mg/L |
48h-EC50 |
> 100 mg/L |
> 119.1 mg/L |
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0 |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
8.7 mg/L |
Yes |
Table 3: Measured Concentrations
Nominal Concentration |
Test Item Measured concentration t = 0 h |
Measured concentration t = 48 h |
% of nominal concentration t = 0 h |
% of nominal concentration t = 48 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
< LOQ/LOD |
< LOQ/LOD |
-- |
-- |
100 |
116.29 |
122.00 |
116 |
122 |
LOQ (Limit of quantification) = 0.05 mg/L
LOD (Limit of determination) = 0.05 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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