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EC number: 219-547-3 | CAS number: 2459-10-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trimethyl benzene-1,2,4-tricarboxylate
- EC Number:
- 219-547-3
- EC Name:
- Trimethyl benzene-1,2,4-tricarboxylate
- Cas Number:
- 2459-10-1
- Molecular formula:
- C12H12O6
- IUPAC Name:
- 1,2,4-trimethyl benzene-1,2,4-tricarboxylate
- Details on test material:
- - Name of test material (as cited in study report): 1,2,4-Trimethylbenzenetricarboxylate
- Physical state/ Appearance: liquid, viscous/ amber
- Homogeneity: homogeneous
- Analytical purity: 98.6 area-% (233 nm); 99.15 area-% (207 nm) (see analytical report 13L00246)
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed. The samples were transported to the Analytical Laboratory on the day of sampling.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To facilitate handling, the test substance was melted by warming the container in a drying oven to 100°C for about 5 min before use. The test substance was homogenized by shaking the container. The stock test solution (120 mg/L) was prepared by directly adding 120.20 mg of test substance to 1L of test medium and stirring for about one day until visibly dissolved. The stock solution was checked for complete dissolution of the test substance. All test solutions were visibly clear over the exposure period.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.45 mmol/L
- Test temperature:
- 19.9 – 20.4 °C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 8.3 - 8.5 mg/L
- Nominal and measured concentrations:
- nominal concentration: control, 120 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: 207 – 743 lux at a wave length of 400 - 750 nm - Reference substance (positive control):
- yes
- Remarks:
- Sodium chloride
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The EC50(48h) of the reference substance sodium chloride was 5.13 g/L
Any other information on results incl. tables
Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
In a 48-hour acute toxicity study, neonate Daphnia magna were exposed to 1,2,4- Trimethylbenzenetricarboxylate at nominal concentrations of 0 (control), 120 mg/L under static conditions in accordance with the OECD 202 guideline. The water pH, temperature
and dissolved oxygen were within acceptable guideline specifications. Immobilization (mortality) and sublethal effects were observed daily. The 48 hour EC50 values determined in this acute toxicity study were: >120 mg/L based on the nominal concentration of the test
substance and >111 mg/L based on the mean measured concentrations. The test substance had no observable acute effect on Daphnia magna at 120 mg/L All measured concentrations deviated less than 20% from the nominal concentration. Thus, the test substance was stable in solution under test conditions and nominal concentrations are an accurate representation of exposure levels maintained throughout the test period. Following recommendations in OECD 202 the results should be evaluated based on the nominal concentrations. The toxicity results presented here are consistent with the results from preliminary tests. The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study. No deviations from test guidelines or other incidents occurred during the course of the reported test which may have influenced the results.
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