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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Trimethyl benzene-1,2,4-tricarboxylate
EC Number:
219-547-3
EC Name:
Trimethyl benzene-1,2,4-tricarboxylate
Cas Number:
2459-10-1
Molecular formula:
C12H12O6
IUPAC Name:
1,2,4-trimethyl benzene-1,2,4-tricarboxylate
Details on test material:
- Name of test material (as cited in study report): 1,2,4-Trimethylbenzenetricarboxylate
- Physical state/ Appearance: liquid, viscous/ amber
- Homogeneity: homogeneous
- Analytical purity: 98.6 area-% (233 nm); 99.15 area-% (207 nm) (see analytical report 13L00246)

Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed. The samples were transported to the Analytical Laboratory on the day of sampling.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To facilitate handling, the test substance was melted by warming the container in a drying oven to 100°C for about 5 min before use. The test substance was homogenized by shaking the container. The stock test solution (120 mg/L) was prepared by directly adding 120.20 mg of test substance to 1L of test medium and stirring for about one day until visibly dissolved. The stock solution was checked for complete dissolution of the test substance. All test solutions were visibly clear over the exposure period.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.45 mmol/L
Test temperature:
19.9 – 20.4 °C
pH:
7.7 - 8.0
Dissolved oxygen:
8.3 - 8.5 mg/L
Nominal and measured concentrations:
nominal concentration: control, 120 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: 207 – 743 lux at a wave length of 400 - 750 nm
Reference substance (positive control):
yes
Remarks:
Sodium chloride

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50(48h) of the reference substance sodium chloride was 5.13 g/L

Any other information on results incl. tables

Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

In a 48-hour acute toxicity study, neonate Daphnia magna were exposed to 1,2,4- Trimethylbenzenetricarboxylate at nominal concentrations of 0 (control), 120 mg/L under static conditions in accordance with the OECD 202 guideline. The water pH, temperature

and dissolved oxygen were within acceptable guideline specifications. Immobilization (mortality) and sublethal effects were observed daily. The 48 hour EC50 values determined in this acute toxicity study were: >120 mg/L based on the nominal concentration of the test

substance and >111 mg/L based on the mean measured concentrations. The test substance had no observable acute effect on Daphnia magna at 120 mg/L All measured concentrations deviated less than 20% from the nominal concentration. Thus, the test substance was stable in solution under test conditions and nominal concentrations are an accurate representation of exposure levels maintained throughout the test period. Following recommendations in OECD 202 the results should be evaluated based on the nominal concentrations. The toxicity results presented here are consistent with the results from preliminary tests. The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study. No deviations from test guidelines or other incidents occurred during the course of the reported test which may have influenced the results.