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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Pathways of Photoprocessing Chemicals in Publicly Owned Treatment Works
Year:
1977
Bibliographic source:
NATIONAL ASSOCIATION OF PHOTOGRAPHIC MANUFACTURERS. INC.

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: modified provisional algal assay procedure (PAAP)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(2-(4-amino-N-ethyl-m-toluidino)ethyl)methanesulphonamide sesquisulphate
EC Number:
247-161-5
EC Name:
N-(2-(4-amino-N-ethyl-m-toluidino)ethyl)methanesulphonamide sesquisulphate
Cas Number:
25646-71-3
Molecular formula:
C12H21N3O2S.3/2H2O4S
IUPAC Name:
Methanesulfonamide, N-[2-[(4-amino-3-methylphenyl)ethylamino]ethyl]-, sulfate (2:3)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Basic ASM Medium, sterile distilled water, and test chemical were dispensed into 600 mL Erlenmeyer flasks
- Controls: Yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Green algae
- Strain: Selenastrum capricornutum, number 1648
- Source: Slant agar cultures obtained from the Indiana University culture collection
- Age of inoculum (at test initiation): Seven day-old stock cultures were used for each inoculation. A portion of the stock culture was analyzed for chlorophyll "a", dry weight, and absorbance at 750 nm immediately prior to use in the algal assay.
- Method of cultivation: New stock cultures were prepared at weekly intervals by inoculating approximately 500 mL of fresh Basic ASM Medium with 10 mL of algal suspension.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Test temperature:
20 °C ± 1 °C
pH:
7.0
Nominal and measured concentrations:
Control, 0.4, 4.0 and 40 mg/L
Details on test conditions:
TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: 600 mL Erlenmeyer flasks, 10 mL of algal suspension and brought up to a final volume of 500 mL with basic ASM medium
- Aeration: Diffused aeration from a compressed air supply, after three-stage filtration, which included final passage through sterile cotton, no stirring or shaking.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: Yes (ASM medium but not standard for algae toxicity testing according to OECD 201)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Basic ASM Medium; Stock solutions of the test item used in formulating the final medium were prepared from reagent grade chemicals, dissolved in distilled water, and sterilized by passage through a 0.45 µm Millipore filter. All glassware utilized in the algal assays were successively washed with several portions each of 6N HCl, 70% isopropanol, and Millipore filtered distilled water.

OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Adjustment of pH: Yes, 7.0
- Photoperiod: Continuous overhead illumination
- Light intensity and quality: Two 40 watt Sylvania soft-white fluorescent tubes, suspended 18 inches from the flasks (corresponding to approximate light intensity of 500 foot-candles)

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Measurement of absorbance at 750 nm.
- Other: Algal assays were conducted over a period of seven days. At the end of each experiment, additional samples were taken for analysis of dry weight and chlorophyll "a".

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
Range finding study: No range-finding test was performed
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
> 0.4 - < 4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield

Any other information on results incl. tables

Table 1: Biological Results based on 7 -day Cell yield and Growth Rate constant

Conc

7-day cell yield

Growth rate

Effect

[mg/L]

% Difference from Control

95% confidence Interval (Controls)

% Difference from Control

95% confidence Interval (Controls)

 

0.4

+0.5

±52.7

78.0

±40.5

N

4.0

-69.9

±52.7

50.3*

±40.5

I

40.0

-92.1

±52.7

12.1*

±40.5

I

I: Inhibition; N = No effect

* due to imprecise at this adsorption range these values are not reliable

Applicant's summary and conclusion