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EC number: 200-624-5 | CAS number: 66-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- no
- Specific details on test material used for the study:
- no details given
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: five concentrations, geometrically increasing with a factor of 1.8
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- no details given
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: guppy
- Source: laboratory-reared
- Age at study initiation (mean and range, SD): 2-3 months
- Feeding during test : yes
- Food type: commercial food (TetraPhyll, Tetra Werke, Germany)
- Frequency: daily
ACCLIMATION
- Acclimation period: at least 12 days
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 21 - 23°C
- pH:
- 6.5 -7.5
- Dissolved oxygen:
- Oxygen content was sometimes found to be very low, approx. 3 mg/L, 24 h after preparation of the solutions
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- not specified
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessels
- Size of vessel: 1.5 L
- Type (delete if not applicable): closed with glass lids
- Material, size, headspace, fill volume: 1.4 L
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of vessels per concentration (replicates): 10 fish per concentration
- Control: control fish were exposed to 72 µl/L acetone,
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 8 times daily (4 times before and 4 times after water renewal)
OTHER TEST CONDITIONS
- Photoperiod: 12h/12h (light/dark)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- no
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 97.7 µmol/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Remarks:
- The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Remarks:
- calculated based on the MW of 100.16 g/mol
- Effect conc.:
- 9.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.
- Basis for effect:
- mortality
- Details on results:
- no details given
- Results with reference substance (positive control):
- not specified
- Reported statistics and error estimates:
- LC50 data were calculated by logit transformation.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- An LC50 (14 d) of 9.8 mg/L was determined in P. reticulata under semi-static conditions.
- Executive summary:
A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.
Reference
Description of key information
A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.
Key value for chemical safety assessment
Additional information
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