Hexanal

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

GLP compliance:

no

Specific details on test material used for the study:

no details given

Analytical monitoring:

yes

Details on sampling:

- Concentrations: five concentrations, geometrically increasing with a factor of 1.8

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

no details given

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: guppy
- Source: laboratory-reared
- Age at study initiation (mean and range, SD): 2-3 months
- Feeding during test : yes
- Food type: commercial food (TetraPhyll, Tetra Werke, Germany)
- Frequency: daily

ACCLIMATION
- Acclimation period: at least 12 days

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Test temperature:

21 - 23°C

pH:

6.5 -7.5

Dissolved oxygen:

Oxygen content was sometimes found to be very low, approx. 3 mg/L, 24 h after preparation of the solutions

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

not specified

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Size of vessel: 1.5 L
- Type (delete if not applicable): closed with glass lids
- Material, size, headspace, fill volume: 1.4 L
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of vessels per concentration (replicates): 10 fish per concentration
- Control: control fish were exposed to 72 µl/L acetone,

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 8 times daily (4 times before and 4 times after water renewal)

OTHER TEST CONDITIONS
- Photoperiod: 12h/12h (light/dark)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Reference substance (positive control):

no

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

97.7 µmol/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.

Basis for effect:

mortality

Key result

Duration:

14 d

Dose descriptor:

LC50

Remarks:

calculated based on the MW of 100.16 g/mol

Effect conc.:

9.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

The concentrations used in the calculations were based on the added amount of material. The resulting LC50 was then corrected for loss of the compound through evaporation etc. by multiplication with its recovery factor.

Basis for effect:

mortality

Details on results:

no details given

Results with reference substance (positive control):

not specified

Reported statistics and error estimates:

LC50 data were calculated by logit transformation.

Validity criteria fulfilled:

not specified

Conclusions:

An LC50 (14 d) of 9.8 mg/L was determined in P. reticulata under semi-static conditions.

Executive summary:

A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.

Description of key information

A long-term toxicity (14 d) assay on the guppy (Poecila reticulata) was conducted with the test item hexanal simliar to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 5 analytically verified concentrations were tested on 10 fish per concentration under semi-static conditions. The LC50 after 14 d was determined by logit transformation. The study is well documented and meets generally accepted scientific principle and is therefore sufficient for the assessment. An LC50 (14 d) value of 97.7 µmol/L, which corresponds to 9.8 mg/L was derived.