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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
yes
Remarks:
Study predates development of OECD Test Guidelines
GLP compliance:
no
Remarks:
Study predates the establishment of GLP guidelines
Test type:
fixed concentration procedure
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Triisononyl benzene-1,2,4-tricarboxylate
EC Number:
258-847-9
EC Name:
Triisononyl benzene-1,2,4-tricarboxylate
Cas Number:
53894-23-8
Molecular formula:
C36H60O6
IUPAC Name:
triisononyl benzene-1,2,4-tricarboxylate
Test material form:
liquid
Details on test material:
Production sample
Specific details on test material used for the study:
One large bottle of the test substance was received from Esso Research and Engineering Company on March 7, 1969. The test substance was described as a light gold liquid.

Test animals

Species:
rat
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
inhalation: aerosol
Type of inhalation exposure:
whole body
Vehicle:
clean air
Details on inhalation exposure:
The exposure was conducted in a 1000-liter stainless steel and plexiglas chamber under dynamic conditions. The experimental atmosphere was generated by metering the compound with a Precision dual syringe feeder into a positive pressure spray nozzle situated within the chamber. The chamber airflow was maintained by a positive pressure rotary pump located on the exhaust side of the chamber and was monitored by a rotameter. The nominal concentration of test material in the experimental atmosphere was calculated from the ratio of the weight of compound aerosolized to the total chamber airflow (volume of air ejected from the spray nozzle plus the volume of makeup air) per unit time. During exposure, the animals were housed individually in stainless steel exposure baskets, centered in the chamber on a rack. They were observed for signs of irritation, toxicity, or death.

The specific gravity of the test substance was determined to be 0.96 g/mL. The test substance was metered into the chamber and aerosolized at the rate of 9.0 mL/minute (8.64 grams/minute). Airflow through the chamber was maintained at 154 L/min. Thus, the nominal concentration of MRD-69-31 in the chamber atmosphere was calculated to be 56 mg/L air.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
ca. 6 h
Concentrations:
56 mg/L
No. of animals per sex per dose:
10
Control animals:
yes
Details on study design:
Six male animals of each species were exposed to filtered room air under experimental conditions to serve as controls. After exposure, the animals were removed from the chamber and group housed according to species. They were observed daily for 14 days for latent toxic effects. Necropsies were performed on all animals. The lungs, trachea, liver, and kidneys were removed, examined for gross pathological signs, and preserved in 10% buffered formalin for possible future histopathological examination.

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC0
Effect level:
>= 56 mg/L air (nominal)
Based on:
test mat.
Exp. duration:
6 h
Mortality:
No mortality occured in rats
Clinical signs:
other: During exposure altered respiratory patterns consisting of slow and irregular breathing were observed. Restlessness, partial eye closure, and rapid respiration were noted in most of the animals within five minutes after initiation of exposure. After 15 mi
Body weight:
No significant effects were noted. Initial average rat body weight of 253 grams increased to 311 grams at termination.
Gross pathology:
Necropsy findings showed compound-related effects in the areas of discoloration in lungs and discoloration of the renal medullae.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
No test substance related mortality occurred under the study conditions.
Executive summary:

A group of 10 male rats (strain not specified) were exposed of a 56 mg/L aerosol of the test substance for six hours and subsequently observed for up to 14 days. No test substance-related mortality occured. Gross necropsy findings included some discoloration of the lung and kidney (renal medullae).