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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11/12/2017 - 18/05/2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: UpChem Co. Ltd., 170329
- Expiration date of the lot/batch: 28-03-2018
- Purity test date: 06-04-2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature, away from moisture
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: not soluble
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not observed
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna was used as test system as it is a recommended species in the guideline.
6.1.2.1 Source
Ecotoxicology
Eurofins Advinus Limited
Bengaluru
India
6.1.2.2 Maintenance of Daphnia
Daphnia were maintained in the test medium. The test medium in which the daphnia were maintained was changed at least twice a week and they were fed with the unicellular green alga, Chlorella vulgaris.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Reference substance (positive control):
yes
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
The EC50 value for P-phenylene diisocyanate at 48 hours was 4.46 mg/L based on nominal concentrations with fiducial limits at 95 % being 3.1463 and 6.3208 mg/L.
Executive summary:

The acute immobilization effect of the test item P-phenylene diisocyanate was studied onDaphnia magna. TheDaphnia magnaof less than 24 hours old were exposed to concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L of P-phenylene diisocyanate along with a negative and reference substance in reconstituted water at 20 ± 1oC. The number of daphniaimmobilized was recorded at 24 and 48 hours post exposure.

 

There was no immobilization of daphnia in the negative control and at the lowest concentration of 0.4 mg/mL either at 24 or at 48 hours post-exposure. The immobilization of daphniawas 5, 5, 35, 35, 55, 85 and 100 % at 24 hours and 15, 20, 45, 80, 85, 95 and 100 % at 48 hours post-exposure at the tested concentrations of 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L ofP-phenylene diisocyanate.

 

There was 40 and 60% immobilization of daphnia in thereference substance (potassium dichromate), at 24 and 48-hour post exposure, respectively, indicating the sensitivity of the test procedure used.

 

The EC50value for P-phenylene diisocyanate at 24 hours was 11.626 mg/L based on nominal concentrations with fiducial limits at 95 % being 7.7588 and 17.4207 mg/L.

 

The EC50value for P-phenylene diisocyanate at 48 hours was 4.46 mg/L based on nominal concentrations with fiducial limits at 95 % being 3.1463 and 6.3208 mg/L.

Description of key information

The EC50 value for P-phenylene diisocyanate at 48 hours was 4.46 mg/L based on nominal concentrations with fiducial limits at 95 % being 3.1463 and 6.3208 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
4.46 mg/L

Additional information