Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-207-6
CAS number: 104-49-4
The effect of
P-phenylene diisocyanate was tested on the growth of freshwater single
cell green algaPseudokirchneriella subcapitata.
Range finding test was
carried out to select the test concentrations for the definitive test.
In the range finding test, the tested concentration was 0.00001, 0.0001,
0.001, 0.01, 0.1, 1, 10 and 100 mg/L with single replicate. The test
item exhibited 15.91, 0.65, 2.92, 10.39, 20.45, 33.44, 51.30 and 100 %
reduction in cell biomass at the tested concentrations of 0.00001,
0.0001, 0.001, 0.01, 0.1, 1, 10 and 100 mg/L at 72 h.
The alga was exposed to
the test item at the nominal concentrations of 0.4, 1.2, 3.6, 10.8, 32.4
and 97.2 mg/L (factor of about 3) along with a negative control and a
reference substance (Potassium dichromate) at 0.9 mg/L. The cell growth
was measured at 24, 48 and 72 hours after the initiation of the test.
concentration/effect relationship was determined using two factors
namely growth rate and yield at the end of the test (72 hours). The per
cent inhibition of growth rate was 1.65, 4.94, 12.37, 15.03, 45.35 and
100% and the percent yield inhibition was 6.99, 19.53, 42.06, 48.50,
87.13 and 100% at the nominal concentrations of 0.4, 1.2, 3.6, 10.8,
32.4 and 97.2 mg/L,
respectively, when compared to the negative control. There was 23.34 %
and 64.48 % inhibition of average growth rate and yield of alga,
respectively, in the reference substance group at 0.9 mg/L. Observed
cells were found morphologically normal.
The EC values
calculated for growth rate and yield on the basis of the nominal
concentration of the test item are presented below:
At 72 hours
EC values (mg/L)
ErCrefers to growth rate and EyC refers to yield
The EC50 value calculated for growth rate is 25.46 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again