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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane
EC Number:
Molecular formula:
Al, MgO, Mg(AlO2)2
dioxo-2,4-dioxa-1,5-dialumina-3-magnesapentane oxomagnesium alumane
Details on test material:
Content: aluminium 708,000 mg/kg, magnesium 68,000 mg/kg, ammonium 22,000 mg/kg, and other ingredients
Batch number: Delivery of 21.04.2009
Expiry date: 2011-08-21

Sampling and analysis

Analytical monitoring:
Details on sampling:
Examined at 0 and 48 hours.

Test solutions

Details on test solutions:
Direct weighings were prepared to give the desired series of test concentrations. 21.9, 47.9, 105.6, 231.6 and 509.7 mg of the test item were added each to 1 litre of dilution water, treated for 1 h in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using folded filters of pore size 7-12 μm. The pH of each test solution was adjusted to 6.0.
19 mL of the solutions were taken and diluted with 1 mL of dilution water containing 10 daphnids resulting in final concentrations of 20.7, 45.5, 100, 220 and 484 mg/L. For each test item concentration and the control 2 replicates were prepared.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
48 h

Test conditions

Test temperature:
20.0 to 21.0 °C
Nominal and measured concentrations:
Nominal: 20.7, 45.5, 100, 220 and 484 mg/L
Details on test conditions:
Experimental design : 5 test concentrations plus 1 control, 10 neonates per vessel, 2 replicates per concentration/control, no feeding during the exposure period
Photoperiod : 16 h light : 8 h dark
Reference substance (positive control):
not required

Results and discussion

Effect concentrationsopen allclose all
48 h
Dose descriptor:
Effect conc.:
> 484 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
24 h
Dose descriptor:
Effect conc.:
> 484 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
The 48h-EC50 is expressed in term of actual loading of Aluminium-Skimmings.

The highest test concentration (484 mg/L nominally, corresponding to a mean measured concentration of 3.5 mg/L) exhibited a 20 % immobilisation effect after 24 h and 48 h of exposure.

Any other information on results incl. tables

Chemical analysis

Control  0h  48h

 Aluminium determination (two replicates) [mg/L]

  0.0082 / 0.0083  0.0017 / 0.0016

 Aluminium determination (mean value) [mg/L]

  0.0083 0.0017

The recoveries of Al at concentrations slightly higher than the Limit of Quantitation (1.0 μg/L) are not considered critical with respect to control performance.

100 mg/L  0h  48h

 Aluminium determination (two replicates) [mg/L]

  0.2027 / 0.2072  0.0862 / 0.0837

 Aluminium determination (mean value) [mg/L]

 0.2050 0.0850

220 mg/L  0h  48h

 Aluminium determination (two replicates) [mg/L]

 0.2161 / 0.2198 2.7922/ 2.8584

 Aluminium determination (mean value) [mg/L]

0.218 2.8253

484 mg/L  0h  48h

 Aluminium determination (two replicates) [mg/L]

 3.4998/ 3.5048 0.1218 / 0.1271

 Aluminium determination (mean value) [mg/L]

 3.5023 0.1245

Mean recovery rate (100, 220, 484 mg/L) at 0 h in %        0.34

Mean recovery rate (100, 220, 484 mg/L) at 48 h in %       0.24

Applicant's summary and conclusion

Executive summary:

The acute toxicity of Aluminia Skimmings towards daphnids is characterized by an EC50(48h) of >484 mg/L, (Currenta, 2010).