Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 January 2018 - 22 March 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

HPLC-MS/MS

Details on sampling:

- Nominal Concentrations: 1.0, 1.8, 3.2, 5.6 and 10 mg/L.
- Geometric Mean Measured Test Concentration (mg/L): 0.61, 1.6, 2.8, 5.7 and 9.7
- Sample storage conditions before analysis: All samples were stored frozen prior to analysis

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-A nominal amount of test item (50 mg) was dissolved in test water and the volume adjusted to 500 mL to give the 100 mg/L test concentration from which a series of dilutions was made to give further test concentrations of 0.10, 1.0 and 10 mg/L.

Based on the results of the range-finding test the following test concentrations were assigned to the definitive test: 1.0, 1.8, 3.2, 5.6 and 10 mg/L.
A nominal amount of test item (20 mg) was dissolved in test water and the volume adjusted to 2 liters to give the 10 mg/L test concentration from which a series of dilutions was made to give further test concentrations of 1.0, 1.8, 3.2 and 5.6 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory cultures
- Feeding during test: none

ACCLIMATION
- Type and amount of food:mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension.
- Feeding frequency: daily

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not specified

Test temperature:

18 to 22 °C with a maximum deviation of ±1 °C

pH:

7.7 - 8.2

Dissolved oxygen:

8.2 - 9.2 mg /L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

- Nominal Concentrations: 1.0, 1.8, 3.2, 5.6 and 10 mg/L.
- Geometric Mean Measured Test Concentration (mg/L): 0.61, 1.6, 2.8, 5.7 and 9.7

Details on test conditions:

TEST SYSTEM
- Type: closed
- No. of organisms per vessel: 5
- replicates: 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.
- Light intensity: between 200 and 1200 Lux

RANGE-FINDING STUDY
- Test concentrations: 0.10, 1.0, 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.61 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 1.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Abnormal responses: A single daphnia was observed to have reduced movement in the control group at 24 hours however, this was considered not to have had an impact on the outcome of the test given that no more than 10% were affected.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50 : 24hours 0.79mg/L and 48 hours 0.75mg/L,
- NOEC: 24 hours 0.56 mg/L and 48 hours 0.56 mg/L
- LOEC: 24 hours 1.0 mg/L and 48 hours 1.0 mg/L
- Other: potassium dichromate as the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L

Reported statistics and error estimates:

not specified

Validity criteria fulfilled:

yes

Remarks:

The test was considered to be valid given that no more than 10% of the control daphnids showed immobilization or other signs of disease or stress

Conclusions:

48 hours: EC50 = 1.1 mg/L, NOEC = 0.61 mg/L, LOEC = 1.6 mg/L

Description of key information

Daphna Magma, 48 hours:

EC50 = 1.1 mg/L,

NOEC = 0.61 mg/L,

LOEC = 1.6 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.1 mg/L

Additional information

A determination of the acute toxicity to aquatic invertebrates Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts was conducted under semi-static test conditions (Envigo Research Limited, 2018). The study was performed in accordance with EC method C2 and OECD test guideline 202, and in compliance with GLP. Groups of 20 Daphnia magna were exposed to 0 (control group), 0, 0.61, 1.6, 2.8, 5.7 and 9.7 mg/L (geometric mean measured concentration) Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts in Elendt M7 Culture medium.

After 48 hours, 95% of the Daphnia in the 1.6, and all 2.8, 5.7 and 9.7 mg/L concentration groups were immobilised. This response was reduced mobility and covered with debris. None of the Daphnia in the lower concentration group were immobilised. A single daphnia was observed to have reduced movement in the control group at 24 hours however, this was considered not to have had an impact on the outcome of the test given that no more than 10% were affected

It was concluded that the 48 hour EC50 in Daphnia Magna was 1.1 mg/L. The no-observed effect concentration was concluded to be 0.61 mg/L.