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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)
EC Number:
238-200-7
EC Name:
Tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)
Cas Number:
14285-59-7
Molecular formula:
C32H16CoN8O12S4
IUPAC Name:
tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Number of animals: 20 animals in test group and control group respectively, divided into 4 replicates (5 animals / replicate)
Age at test start: < 24 hours old

Study design

Test type:
semi-static
Water media type:
other: Reconstituted water (ISO medium, according to OECD 202)
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
249 mg/L
Test temperature:
Test temperature in the test vessels: 20.5 – 20.8°C
Temperature in the climate chamber: 20.4 – 21.1°C.
pH:
pH = 7.36 – 7.54
Dissolved oxygen:
Dissolved oxygen ranges: 8.2 – 8.6 mg/L
Nominal and measured concentrations:
The nominal concentration of 100 mg/L was determined according to a range-finding test.
Corresponding measured concentration: 91.1 mg/L
As the measured concentration deviated not more than 20% from the nominal, the biological results are based on the nominal concentration.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
After 24 and 48 hours of exposure, no effects on mobility were observed at the tested concentration of 100 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No effects on mobility were observed in a limit test on daphnids. The EC50 and the NOEC of the test item are > 100 mg/L.

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