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Registration Dossier
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EC number: 238-200-7 | CAS number: 14285-59-7
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)
- EC Number:
- 238-200-7
- EC Name:
- Tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)
- Cas Number:
- 14285-59-7
- Molecular formula:
- C32H16CoN8O12S4
- IUPAC Name:
- tetrahydrogen [29H,31H-phthalocyanine-2,9,16,23-tetrasulphonato(6-)-N29,N30,N31,N32]cobaltate(4-)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Number of animals: 20 animals in test group and control group respectively, divided into 4 replicates (5 animals / replicate)
Age at test start: < 24 hours old
Study design
- Test type:
- semi-static
- Water media type:
- other: Reconstituted water (ISO medium, according to OECD 202)
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 249 mg/L
- Test temperature:
- Test temperature in the test vessels: 20.5 – 20.8°C
Temperature in the climate chamber: 20.4 – 21.1°C. - pH:
- pH = 7.36 – 7.54
- Dissolved oxygen:
- Dissolved oxygen ranges: 8.2 – 8.6 mg/L
- Nominal and measured concentrations:
- The nominal concentration of 100 mg/L was determined according to a range-finding test.
Corresponding measured concentration: 91.1 mg/L
As the measured concentration deviated not more than 20% from the nominal, the biological results are based on the nominal concentration.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 and 48 hours of exposure, no effects on mobility were observed at the tested concentration of 100 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No effects on mobility were observed in a limit test on daphnids. The EC50 and the NOEC of the test item are > 100 mg/L.
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