5-octylbicyclo[2.2.1]hept-2-ene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 to 20 July 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Temperature range in the incubator recorded during the test was 19.6 - 20.9°C, 0.3°C above the recommended variation limit.

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Materia, Inc.; RP367_ONB-D
- Expiration date of the lot/batch: Retest date 9 August 2018

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient (15±10°C), in the dark
- Stability under test conditions: Not known
- Solubility and stability of the test substance in the solvent/vehicle: Not known

OTHER SPECIFICS:
Purity/Composition: ONB Isomers (2) 93.26%; TCPD Isomers (3 major) 3.92%; Other individual impurities < 0.99% ea; (Taken from Certificate of Analysis)
Chemex reference: ECO 180212
Appearance (observed): Colourless liquid
Solubility (specified): <0.001g/L
Homogeneity (observed): Homogeneous
Date of receipt: 15 February 2018

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Nominal test concentrations: 0 (control) and 100mg/l; Initial measured test concentrations: 0 (control) and 4.562mg/l
- Sampling method: Chemex SOP E201 based on the OECD 202 guideline Daphnia sp. Acute Immobilisation Test.
- Sample storage conditions before analysis: Ambient (15±10°C), in the dark

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Not provided
- Source: From cultures maintained at Chemex Environmental International Ltd since July 2014 and originally sourced from a clone of genotype 5.
- Age of parental stock (mean and range, SD): Not provided
- Feeding during test: The Daphnia in the test were not fed.
- Food type: Not provided
- Amount: Not provided
- Frequency: Not provided

- Date of gravid isolation: 18 July 2018
- Age of Daphnia at start of test: ≤ 24 hours.
- Culture conditions: Cultured under static conditions
- Temperature: 20 ± 2°C
- Dissolved oxygen: >60% (or 3mg/l) ASV
- Photoperiod: 16 hours light and 8 hours dark
- Feeding regime: Each working day, a suspension of Chlorella vulgaris. The Daphnia in the test were not fed.

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

Test duration 48+/-1 hour

Test temperature:

Temperature range within incubator throughout test: 19.6 - 20.9°C

pH:

Initial pH at 0 Hours: 6.92
pH range in control and test concentrations throughout test: 6.81 - 7.46

Dissolved oxygen:

Initial Dissolved Oxygen at 0 Hours: 6.98mg/l
DO range in control and test concentrations throughout test: 6.08 - 7.13mg/l

Nominal and measured concentrations:

Nominal test concentrations: 0 (control) and 100mg/l
Initial measured test concentrations: 0 (control) and 4.562mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 40ml glass dishes. To limit aerial contamination of the test vessels and reduce evaporative losses, the vessels were covered with a perspex sheet.
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: Test volume: 25ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 reconstituted Daphnia water
- Intervals of water quality measurement: The pH (to 0.01), dissolved oxygen mg/l (to 0.01) and temperature (to 0.1°C) measurements were taken at 0 hours on remaining test solution, and at 48-hour endpoint on pooled replicates for each test and control solution. Temperature of incubation was recorded at 0, 24 and 48 hours.

OTHER TEST CONDITIONS
- Light intensity: Illumination: 16 Hours Light, 8 Hours Dark Cycle

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The number of immobilised Daphnia was recorded after 24 and 48-hour exposure periods. Where possible, the EC(r)10, EC(r)50 EC(r)90 values with 95% confidence limits and NOEC(r) were calculated according to the statistical methods of ToxCalc™ Version 5.0 “Comprehensive Toxicity Data Analysis and Database Software”, copyright 1994-1996.

VEHICLE CONTROL PERFORMED: No

RANGE-FINDING STUDY
- Test concentrations: 0 (control), 0.1, 1, 10 and 100mg/l.
- Results used to determine the conditions for the definitive study: 48-hour EC50 >100mg/l.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (Study in 2017).

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 4.562 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight: No details provided
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: The Daphnia were examined at 24-hours and 48-hours for abnormal behaviour during the determination of immobility. All Daphnia appeared normal with no signs of abnormality.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None

No test concentration immobilised all twenty Daphnia after 48-hours. The highest concentration where no immobilisation occurred was 4.562mg/l.

Results with reference substance (positive control):

A separate GLP study (ENV 11395) was conducted from 18 to 20 January 2017 to monitor the sensitivity of the test animals and test procedures. The reference study gave a 24 hour EC50 of 1.4mg/l of potassium dichromate which was within the acceptable range 0.6 mg/l to 2.1 mg/l as specified in Technical Corrigendum to ISO6341.

Analytical measurement of the freshly prepared exposure solutions showed that the mean measured concentrations were not within 20% of nominal concentrations. However, analytical measurement of the aged exposure solutions showed that all of the concentrations were within 20% of the initial measured concentrations. Therefore in accordance with OECD Guidelines, the initial measured concentrations have been used in the determination of the EC50, NOEC and LOEC values.

Validity criteria fulfilled:

yes

Conclusions:

48h EC50 >4.562mg/l (Confidence limits not possible to determine).
48h NOEC >4.562mg/l
48h LOEC >4.562mg/l

Description of key information

Analytical measurement of the freshly prepared exposure solutions showed that the mean measured concentrations were not within 20% of nominal concentrations. However, analytical measurement of the aged exposure solutions showed that all of the concentrations were within 20% of the initial measured concentrations. Therefore in accordance with OECD Guidelines, the initial measured concentrations have been used in the determination of the EC50, NOEC and LOEC values.

Key value for chemical safety assessment

Marine water invertebrates

Marine water invertebrates

Effect concentration:

4.562 mg/L

Additional information

EC50 >4.562mg/l