Registration Dossier
Registration Dossier
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EC number: 701-016-7 | CAS number: -
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Benzene, C16-24 alkyl derivatives are also known as High Molecular Weight Linear Alkylbenzenes (HiMo LAB). The substance is a UVCB. Because of their high molecular weight, they are very similar in composition and properties to the heavy alkylate bottoms (HAB). Based on these structural and functional similarities, data on LAB (CAS no: 67774-74-7) being registered under REACH, LAB Alkylate Bottoms (CAS no: 85117-41-5) described in OECD SIDS dossier, and HAB (CAS no: 84961-70-6) being registered under REACH are suitable as supporting studies in case where specific data on the HiMo LAB is lacking.
To assess the genetic toxicity profile of the substance, structral analogue approach was used from HAB (CAS no: 84961-70-6), LAB Alkylate Bottoms (CAS no: 85117-41-5) and Benzene, C10-16 alkyl derivs. (CAS no: 68648-87-3).
The study examined the mutagenic potential of the HAB according to EWG guideline. Salmonella typhimuirum strains TA1538, TA1537, TA1535, TA98, and TA100 were exposed to concentrations of 8, 40, 200, 1000, 5000 ug/plate of test substance in acetone in both the presence and absence of S9. Positive control substances were nitroflourene, sodium azide, or aminoacridine. Cultures were tested in triplicate for mutation frequency. No treatment cultures showed mutation frequencies significantly greater than negative controls. Positive controls showed significantly greater mutation frequencies over negative controls, therefore the test was valid. The test substance is not mutagenic in either the presence or absence of metabolic activation.
The study examined the potential for LAB Alkylate Bottoms to cause mutations according to OECD guideline 473. Chinese hamster ovary (CHO) cells were exposed to concentrations of 5.0-80.0 nL/mL of test substance both in the presence and absence of metabolic activation. After the exposure period, the cells were examined for chromosomal aberrations. Ethylmethanesulfonate and cyclophosphamide were used as positive control substances. No increases in chromosomal aberrations were seen in either the presence or absence of metabolic activation. The test substance is not mutagenic.
The study examined the potential of Benzene,C10-16 alkyl derivs. (Alkylate 225) to cause mutations in mammalian cells according to OECD guideline 476. Chinese hamster ovary cells were exposed to concentrations of 100-2000 microliter/mL of test substance both in the presence and absence of metabolic activation. Ethanol was used as a vehicle. Ethylmethanesulfonate, benzo(a)pyrene, and dimethylnitrosamine were used as positive controls. Cytotoxicity was seen at concentrations of 0.5 mg/mL and above both with and without metabolic activation. No significant increase in mutation frequencies was seen in treatment groups. The test substance is not mutagenic to mammalian cells.
Short description of key information:
No study on mutagenicity is available with the substance. HAB (CAS no: 84961-70-6), LAB Alkylate Bottoms (CAS no: 85117-41-5) and Benzene,C10-16 alkyl derivs. (CAS no: 68648-87-3) were used to cover this endpoint as structural analogue substances. According to the in vitro mutagenicity test results (Ames test, chromosomal aberration test, gene mutation test), the substance is considered to be not mutagenic and does not need to be classified for genotoxicity.
Justification for classification or non-classification
According to the in vitro mutagenicity test results (Ames test, chromosomal aberration test, gene mutation test) from structural analogue substances, the substance is considered to be not mutagenic and does not need to be classified for genotoxicity.
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