Fatty acids, C16-18, reaction products with diethylenetriamine

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

other: read across from analogue substance

Adequacy of study:

key study

Study period:

14 September 2015 - 13 February 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

other: mobile phase

Specific details on test material used for the study:

Test item without emulsifier was investigated.

Amidoamine (UVCB)
Pulcra ID: DE07_2014_012_BEL66 (amidoamine without emulsifier)
Physical state: pale yellowish solid at 20 °C
Batch No.: K8 4309 L481
Expiry date of batch: 09 March 2018
Purity: 100 % (UVCB)
Stability: stable under test conditions
Storage condition of test material: Room temperature, protected from light

Radiolabelling:

no

Test temperature:

30 °C

Details on study design: HPLC method:

The sample, dead time and reference standard solutions were injected in duplicate using the
following HPLC parameters:
HPLC System: Agilent Technologies 1200 Series
Detector types: Ultraviolet (VWD)
Evaporative light scattering (ELSD)
Column: Waters XSelect Cyano, 5 µm (150 x 4.6 mm id)
Column temperature: 30 °C

Mobile phase A: Methanol: water adjusted to pH ~3 using trifluoroacetic acid
Mobile phase B: Methanol: THF

A calibration curve was constructed from the retention time data of the dead time and reference standard solutions. The capacity factors, k', for the reference standards were calculated. A correlation of log k' versus log Koc of the calibration standards was plotted using linear regression.

Analytical monitoring:

yes

Details on sampling:

Test item was diluted to 100 mL with tetrahydrofuran (THF).

Details on matrix:

A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

Key result

Sample No.:

#1

Type:

log Koc

Value:

> 5.63 dimensionless

pH:

3

Temp.:

30 °C

Matrix:

HPLC column containing lipophilic and polar moieties

Key result

Sample No.:

#1

Type:

Koc

Value:

> 427 000 dimensionless

pH:

3

Temp.:

30 °C

Matrix:

HPLC column containing lipophilic and polar moieties

Details on results (HPLC method):

The test system utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used. The dead time was determined by measuring the retention time of formamide (purity: 99.94%, 0.612 g/L solution in mobile phase).
Solutions of reference standards, e.g. Acetanilide, Naphthalene and DDT were prepared in methanol. A calibration curve was constructed from the retention time data of the dead time and reference standard solutions. The mobility classification of the test item was obtained by comparing the calculated Koc
value to the mobility classes according to P.J. McCall, R.L. Swann, D.A. Laskowski, S.M. Unger, S.A. Vrona and H.J. Dishburger “Estimation of Chemical Mobility in Soil from Liquid Chromatography Retention Times”; Bull. Environm. Contam. Toxicol. 24, 190-195 (1980).

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient (Koc) of the test item has been determined to be greater than 4.27 x 10^5, log10 Koc >5.63. These values indicate that the test item will be essentially immobile in the environment.

Executive summary:

The adsorption and desorption of amidoamine test item was determined in a Klimisch 1 GLP study according to EC C.19 and OECD 121. The adsorption coefficient (Koc) of the test item has been determined to be greater than 4.27 x 10^5, log10 Koc >5.63. These values indicate that the test item will be essentially immobile in the environment. This study is considered relevant for the risk assessment.

Description of key information

Analogue substance was tested for adsorption/desorption potential following OECD 121 and LogKoc a t30°C and pH=3 is equal to 5.63 showing high sorptive characteristics and therefore no motility in the environment.

Based on the read across considerations same results can be applied to Stearamide DETA

Key value for chemical safety assessment

Koc at 20 °C:

426 579