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EC number: 948-562-4
CAS number: -
Results after treatment with Reactive Blue F07 -0195 and the controls
viability [rounded values]:
relative viability [rounded values]:
The optical pre-experiment (colour interference pre-experiment) to
investigate the test item’s colour change potential in water resulted in
blue colour due to the intrinsic colour of the test item itself.
Optical evaluation of the MTT-reducing capacity of the test item
after 1 hour incubation with MTT-reagent did showed blue/purple colour.
The mean relative viability of the test
item, corresponding to cell viability, decreased to71.3%
(threshold for irritancy:≤50%),
consequently the test item was declared as non-irritant
vitro study was performed to assess the irritation potential of
Reactive Blue F07-0195 by means of the Human Skin Model Test.
test item reduced MTT (test for direct MTT reduction), and its intrinsic
colour was intensive (test for colour interference). Consequently,
additional tests with freeze-killed and viable tissues were necessary.
three tissues of the human skin model EpiDerm™were
treated with the test item, the negative control (DPBS) or the positive
control (5% SDS) for 60 minutes.
treatment with the negative control the absorbance values were well
within the required range of the acceptability criterion of mean OD³0.8
and ≤ 2.8 for the 60 minutes treatment interval, thus assuring the
quality of the tissues.
with the positive control induced a sufficient decrease in the relative
absorbance as compared to the negative control for the 60 minutes
treatment interval, thus assuring the validity of the test system.
treatment with the test item Reactive Blue F07-0195 the mean relative
viability value decreased to 71.3% compared to the relative absorbance
value of the negative control. This value is above the threshold for
irritancy of ≤ 50%. Therefore, the test item is not considered to
possess an irritant potential.
In conclusion, it can be
stated that in this study and under the experimental conditions
reported, Reactive Blue F07-0195 is non-irritant to skin.
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