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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Title:
Unnamed
Year:
1995

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
(Only 2 strains were tested)
Principles of method if other than guideline:
Method: preincubaton
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium 6-hydroxynaphthalene-2-sulphonate
EC Number:
205-218-1
EC Name:
Sodium 6-hydroxynaphthalene-2-sulphonate
Cas Number:
135-76-2
Molecular formula:
C10H7NaO4S
IUPAC Name:
sodium 6-hydroxynaphthalene-2-sulphonate

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 98, TA 100.
Metabolic activation:
with and without
Metabolic activation system:
-S9 and +S9 were tested.
Test concentrations with justification for top dose:
0-10000 (0, 50, 100, 500, 1000, 5000, 10000) ug/plate were tested
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO

Results and discussion

Test results
Key result
Species / strain:
S. typhimurium, other: TA 98, TA 100,
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The test item did not show any mutagenic effect under test conditions, with and without metabolic activation.

Applicant's summary and conclusion

Conclusions:
The test item did not show any mutagenic effect under test conditions, with and without metabolic activation.
Executive summary:

The Ames test was carried out with the test item using Salmonella typhimurium strains TA 98, TA 100. The test was performed with and without matabolic activation. The concentrations were 0-10000 (0, 50, 100, 500, 1000, 5000, 10000) µg/plate.

The substance did not show any mutagenic effect under test conditions.