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EC number: 908-917-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Mar - 19 Apr 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17. July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted 30. May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations at the beginning and end of the test and at medium renewal (every 24 h) in the new and old test solutions. Samples of the control were taken at test start.
- Sample preparation: To a 100 mL of a solution in matrix 6 g NaCl is added, then, the solution was extracted two times with the solvent dichloromethane (2 * 4 mL), the organic phase was collected into a 10 mL flask, after drying with Na2SO4. The flask was filled up to 10 mL with dichloro-methane after addition of 100 µL ISTD stock solution (100 mg/L) and the solution was measured via GC/MSD. Tenfold enrichment was achieved during extraction procedure.
- Storage: All samples were analysed directly after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To homogenize the test item, it was melted at 40 °C before use. A WAF was prepared by weighing the nominal load of 6 mg/L, adding the corresponding amount of tap water and thoroughly homogenised by stirring for 24 ±1 h. The resulting solution is filtrated through 0.45 µm cellulose ester filters. During membrane filtration of the water-accommodated fraction (WAF), the filters were renewed after filtration of 500 mL solution. Because the test item is not stable under test conditions, the study was performed under semi-static conditions. For medium renewal after 1, 2- and 3-day(s), fresh test solutions were prepared.
- Controls: Medium without test item - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: UBA (Umweltbundesamt, Dessau, Germany) and Dehner Garten Center, 67433 Neustadt, Germany and combined with the offspring of the original animals to one batch.
- Age at study initiation: sexually immature young fish, the offspring of the original animals is used in the test.
- Length at study initiation: 2 ± 1 cm
- Method of breeding: Photo period: 12/12 h, using neon tubes; Temperature: 23 ± 2 °C; Medium: tap water;
ACCLIMATION
- Acclimation period: Before being used for the test, the fish were kept for at least 12 days under test conditions.
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: Warmwater fishfood
- Feeding frequency during acclimation: three times per working day
- Health during acclimation (any mortality observed): During 7 days, no mortality occurred.
FEEDING DURING TEST : no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 0.88 mmol/L
- Test temperature:
- 23.2 - 23.8 °C (Control)
23.1 - 24.4 °C (Test item concentration) - pH:
- 8.1 - 8.0 (Control)
7.9 - 8.1 (Test item concentration) - Dissolved oxygen:
- 7.4 - 9.2 mg/L (Control)
7.7 - 9.1 mg/L (Test item concentration) - Conductivity:
- 210 µS/cm at 25 °C
- Nominal and measured concentrations:
- Control and 6 mg/L (nominal)
0.536 (measured concentration) - Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: glass aquaria, maximal volume 10 L, 7 L test solution, 3 L headspace
- Aeration: Accomplished with glass tubes
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1 vessel
- No. of vessels per control (replicates): 7
- Biomass loading rate: 0.26 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: <0.5 mg/L
- Polycyclic aromatic hydrocarbons: <0.008 µg/L
- Others: Sodium 4.4mg/L, potassium 2.5 mg/L, calcium 32 mg/L, magnesium 2.1 mg/L, aluminium 0.007 mg/L, iron <0.005 mg/L, manganese <0.005 mg/L, ammonium <0.05 mg/L, nitrate 1.4 mg/L, nitrite <0.01 mg/L, chloride 9.2 mg/L, sulphate 10 mg/L, antimony <0.001 mg/L, arsenic <0.001 mg/L, lead <0.001 mg/L, cadmium <0.0001 mg/L, chromium <0.0005 mg/L, copper 0.002 mg/L, nickel <0.001 mg/L, mercury <0.0001 mg/L, selenium <0.001 mg/L, bor <0.01 mg/L, cyanide <0.005 mg/L, fluoride <0.1 mg/L, benzene <0.1 µg/L
- Pesticides and biocides: < LOD mg/L
- Chlorine: chlorinated organic compounds < 1 µg/L
- Culture medium different from test medium: No
- Intervals of quality measurements: Observations were made every 24 h, measuring pH and O2 concentration of the old and new test solution in each vessel and documenting mortalities.
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h
- Light intensity: using neon tubes
EFFECT PARAMETERS MEASURED: Mortality (A fish was considered dead, if no visible movement could be observed, and if touching of the caudal peduncle produced no reaction).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: only one test item concentration
- Range-finder: No Range-finding test was performed - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.536 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.536 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: No - Reported statistics and error estimates:
- No mortality was observed so no statistical evaluation was performed.
- Sublethal observations / clinical signs:
Table 1: Analytical Results: Measured concentrations (purity 26.6 % component 1)
Nominal Concentration Test Item
Measured
Concentration
t = 0 hMeasured
Concentration t = 24 h old
Measured
Concentration t = 24 h new
Measured
Concentration t = 48 h old
Measured
Concentration t = 48 h new
Measured
Concentration t = 72 h old
Measured
Concentration
t = 72 h newMeasured
Concentration
t = 96 h oldmg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
Blank control
< LOD
--
--
--
--
--
--
--
6
0.478
< LOD
0.591
< LOD
0.556
< LOD
0.518
--
LOD = 0.0584 mg/L (based on the lowest calibration level 0.584 and enrichment factor 10)
Table 2: Biological Results: Mortality
Nominal Conc. in mg/L
Total number of fish used
Number of dead fish
24 h
48 h
72 h
96 h
Blank control
7
0
0
0
0
6
7
0
0
0
0
Neither in the treatment nor in the control any mortality or any sign of abnormal behaviour could be observed.
Table 3: Validity criteria
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
No mortality occured
yes
The dissolved oxygen concentration must have been at least 60 per cent of the air
saturation value throughout the test
87.1 %
yes
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Reference
Description of key information
LC50 (96 h): >0.536 mg/L (Danio rerio, OECD 203, Mean measured, Mortality)
Key value for chemical safety assessment
Additional information
One experimental study is available investigating the toxicity of the substance to fish. The study was conducted according to OECD 203 (GLP) using the zebrafish Danio rerio as test species. The test was performed under semi-static conditions at a threshold concentration of 6 mg/L (nominal). The threshhold concentration is based the results of the available experimental data on the acute toxicity to aquatic invertebrates and algae. Due to the low water solubility the test item concentrations were prepared via water-accommodated fractions: 6 mg/L (real load between 6.1 and 6.3 mg/L), adding the corresponding amount of dilution water (tap water) and shaking vigorously for 24 ±1 hours. The resulting solutions were filtrated through 0.45 µm cellulose ester filters. Since the test item was not stable, fresh test solutions were prepared for medium renewal after 1, 2- and 3-day(s). Analytical measurement was done by GC/MSD. The test item consists of two components, ethylene glycol monolaurate (component 1) and ethylene glycol dilaurate (component 2), but only one (component 1) is water soluble. The quantification of the test item was performed using the signal of component 1 only. Due to the test items properties (insufficient stability and adsorbing property), the measured concentration in the aged solution was < LOD for all test item concentrations. Therefore, evaluation was based on the mean measured concentrations in the freshly prepared test solutions. No effects were observed at the mean measured threshold concentration of 0.536 mg/L. Based on these results the fish is not the most sensitive species.
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