Cyclohexadecen-1-one

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and saturated solution
- Sampling method: Samples were taken from freshly
prepared media after 0, 24, 48, and 72 h and from 24 h old media after 24, 48, 72 and 96 h

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The preparation of the saturated solution followed the procedures descirbed in the study "Alga, growth inhibition test with Desmodesmus subspicatus, 72 h" (study no.: SS0115151) (for details s. key study in section 6.1.5). The saturated solution was prepared with the column elution method (quartz sand; 2.1 mg test item/kg quartz sand).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: AQUARIUM AM AEGI, Hannover, Germany
- Length at study initiation: Average body length at the test start: 2.50 cm
- Weight at study initiation: Average body weight at the test start: 0.22 g
- Method of breeding: Holding was performed at the testing facility at 23 ± 2 °C and diffuse light (0.1-10 µmol photons m^-2*s^-1, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value.


ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Stör perlets; SERA GMBH, Heinsberg, Germany; 4 % of the fish body weight per feeding day
- Feeding frequency during acclimation: 3 times per week
- Health during acclimation: no mortality

FEEDING DURING TEST: none

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

57 - 65 mg CaCO3/L

Test temperature:

23.1 - 24.0 °C

pH:

new medium: 7.2 - 8.1
old medium: 7.0 - 7.2

Dissolved oxygen:

new medium: 94 - 100 %
old medium: 61 - 78 %

Nominal and measured concentrations:

Nominal 100% saturated solution
Geometric mean measured concentration: 279 µg/L

Details on test conditions:

TEST SYSTEM
- Renewal rate of test solution: every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin. water was filtered on activated charcola and aerated for at least 24 h to remove chlorine
- Culture medium different from test medium:no
- Intervals of water quality measurement: PH-value, temperature and oxygen saturation were measured in all vessels after beginning the test and every 24 h (from old and new media).

OTHER TEST CONDITIONS
- Photoperiod: natural photoperiod
- Light intensity: 0.1 - 10 µmol photons*m-2*s-1

EFFECT PARAMETERS MEASURED: Symtoms of toxicity and mortality after 2, 24, 48, 72 and 96 h

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 279 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

279 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: s. table
- Mortality of control: none
- Other adverse effects control: none

Sublethal observations / clinical signs:

Observation in the test vessels (n = 7)

Geometric mean measured concentration [µg/L]	Effect*	Test Duration [h]
		2	24	48	72	96
	(1)	-	-	5/7	5/7	6/7
279	(2.4)	7/7	7/7	2/7	2/7	1/7
	(4.1)	7/7	-	-	-	-
Control	(1)	7/7	7/7	7/7	7/7	7/7

*) The number in brackets correspond to the following observation:

(1) = Normal behaviour (2.4) = Slow escape reflex (4.1) = Hyperventilation

Validity criteria fulfilled:

yes

Conclusions:

In this study the test item did not cause lethal effects to zebrafish after 96 h up to the solubility limit (saturation solution level under test conditions: 279 µg/L as geometric mean measured concentration)

Description of key information

The 96 -hour LC50 for Danio rerio > 279 µg/L (solubility limit).

Additional information

The acute toxicity of the test item to Danio rerio was determined according to the principles of the OECD guideline 203 and in compliance with GLP. This test was carried out as a limit test under semi-static conditions with renewal of the test media every 24 hours. Seven test organisms were exposed to a saturated solution of the test item and the control. In this study the test item did not cause lethal effects to zebrafish after 96 h up to the solubility limit (saturation solution level under test conditions: 279 µg/L as geometric mean measured concentration).