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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference Type:
study report

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzyl 2-methoxy-4-prop-1-enylphenyl ether
EC Number:
EC Name:
Benzyl 2-methoxy-4-prop-1-enylphenyl ether
Cas Number:
Molecular formula:
benzyl 2-methoxy-4-prop-1-enylphenyl ether

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Activated sludge was collected from the exit of the aeration tank, or near the exit from the tank, of a well-operated wastewater treatment plant receiving predominantly domestic sewage.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L

Results and discussion

% Degradation
Key result
% degradation (O2 consumption)
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
not readily biodegradable
Percentage of biodegradation at 28 days: 5.9 %
Conclusion: Not readily biodegradable
Executive summary:

The objective of this study was to assess the aerobic ready biodegradability of the test item by measurement of manometric respirometry over a 28-day period according to the OECD 301F

guideline (July 1992).

The test item, at 100 mg.L-1, as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms.

The consumption of oxygen was determined from the change in volume or pressure (or a combination of the two) in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide or another suitable absorbent. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThOD.

As the test item is poorly soluble in test conditions, the test solutions were prepared by direct addition of the test item.

The study is valid since:

Based on a biodegradation rate higher than 60% in 14 days (experimental value: 73.2 % and 76.8 % in 4 days) in the reference control, the test was carried out under good conditions.

The oxygen uptake of the control was not greater than 60 mg.L-1 in 28 days (experimental value: 24.8 mg O2.L-1 and 21 mg O2.L-1).

Based on a biodegradation rate higher than 25% in 14 days (experimental value: 36 % in 13 days) in the toxicity control, the test item was not toxic to micro-organisms.

Any significant Biological Oxygen Demand of the abiotic control over the test period was highlighted. The degradation of test item was not abiotic.

At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did show a difference of more than 20%. This is explained by the low values measured. In fact, this leads to a larger relative difference. This has no impact on the results of the study because the reference control is valid, the toxicity control does not show any toxicity to the microorganisms, and the two replicates show a biodegradability lowest than 20 %.

Results are the following:

Percentage of biodegradation at 28 days: 5.9 %

Conclusion: Not readily biodegradable