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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes (incl. QA statement)
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzyl 2-methoxy-4-prop-1-enylphenyl ether
EC Number:
204-370-6
EC Name:
Benzyl 2-methoxy-4-prop-1-enylphenyl ether
Cas Number:
120-11-6
Molecular formula:
C17H18O2
IUPAC Name:
benzyl 2-methoxy-4-prop-1-enylphenyl ether

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
Positive controls:
yes
Positive control substance:
9,10-dimethylbenzanthracene
9-aminoacridine
2-nitrofluorene
sodium azide
other: 2-aminoanthracene ; cis-Platinum (II) Diammine Dichloride

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
E. coli WP2 uvr A pKM 101
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified

Applicant's summary and conclusion

Conclusions:
Doses (5000, 1500, 500, 150 and 50 µg/plate) performed from solutions of the test item Benzyl Iso Eugenol BATCH: 017448/1 (LEMI code: LM-18/0151), provided by SOCIETE GRASSOISE DE PARFURMERIE do not induce any mutagenic change in Salmonella typhimurium T1535, TA1537, TA98, TA100 and in E. coli WP2(uvrA-) (pKM101) without, or with metabolic activation, according to the OECD Guidelines n°471.