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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD 301 F
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
EC Number:
302-446-4
EC Name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
Cas Number:
94109-09-8
Molecular formula:
C48H84O6
IUPAC Name:
tri(tridecyl) benzene-1,2,4-tricarboxylate
Test material form:
liquid
Details on test material:
- Moisture content: <= 0.1%
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: P-2960F-10
- Expiration date of the lot/batch: November 2013
- Purity test date: > 99%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: no vehicle

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Temperature (°C) at time of collection: incubation temperature 22.1°C +- 0.8°C (deviation)
- Source of activated sludge (e.g. location, contamination history): Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Inoculum Pretreatment: Sludge was centrifuged, supernaturant discarded, and sludge pellet re-suspended in mineral medium twice; sludge was brought up to the original volume; areated in the dark at test temperature until use
- Biomass concentration (mg/L) used in test: 14 mL
Duration of test (contact time):
ca. 28 d
Initial test substance concentration
Initial conc.:
ca. 37 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption

Results and discussion

Test performance:
OECD 301 F
% Degradation
Key result
% Degr.:
ca. 2
Parameter:
O2 consumption
Sampling time:
28 d
Transformation products:
not measured

Applicant's summary and conclusion

Validity criteria
Validity criteria fulfilled:
yes
Conclusions:
Tridecyl Trimellilate did not surpass the 60% biodegradation mark within a 10-day window in a 28 days exposure period, it cannot be considered readily bodegradable.
The test item did not have an adverse effect on the microorganisms in the test.

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